Immunofluorescence

derivation of RBC membrane vesicles and characterize the RBC-membrane-derived vesicles. Specifically, we will: a) Remove the blood group defining antigen using a-galactosidase and 1-N-acetylgalactosaminidase derived enzyme and characterize using immunofluorescence,

Introduction Since the Centers for Disease Control and Prevention starting reporting Lyme disease as a reportable disease in 1991 it has been the most commonly reported vector-borne illness is the United States1. It should be noted that Lyme disease does not occur everywhere in the US, and is heavily concentrated in upper Midwest and northeast United States1. This report has been compiled to provide recommendations for prophylactic treatment of Lyme disease once a patient encounters a tick bite

Development of Optical Imaging Tools for Synapse Typing Restructure of synapse types and alternations of dynamics of neurotransmitters and neuromodulators have been implicated in a number of human neurological and psychiatric diseases, including autism, Alzheimer’s disease, Parkinson’s disease, schizophrenia, and addiction1,2. However, little is known about how molecular compositions define different types and functions of synapses and how different synapses organize in micro- and macroscale to give

INTRODUCTION: Melanoma is a type of cancer that develops in the melanin cells located in the skin. Melanin can cause the pigment of the skin to appear dark when in abundance. Gray horses are especially susceptible due to the abundance of melanin in their skin cells. On the cellular level of the disease, the melanocortin 1 receptor (MC1R) gene provides instructions for making a protein called the melanocortin 1 receptor. This receptor is what allows for normal skin pigmentation and is located of

Malaria is a severe tropical illness spread by mosquitoes. If malaria is not detected and cured quickly, it can be deadly. A lone mosquito bite is the whole thing it takes for somebody to become infected. Malaria is initiated via the plasmodium parasite. The parasite enters to humans after the bites of infected mosquitoes. There are numerous different kinds of plasmodium parasite, however only five forms spread malaria in humans. These are: Plasmodium falciparum, mostly originate in Africa, it is

DUAL DETECTION OF DNA AND LATENT FINGERMARKS FIGURE 1: IDENTIFICATION AND DISTRIBUTION OF ORGANELLES IN A TYPICAL HUMAN CELL. TAKEN FROM CUMMINGS 2015 [2]. 2 FIGURE 2: TYPICAL ARRANGEMENT OF THE PHOSPHOLIPID BILAYER OF A HUMAN CELL. TAKEN FROM CUMMINGS 2015 [2]. 3 1. INTRODUCTION 1.1. FINGERPRINTS 1.1.1. HISTORY 1.1.2. FORMATION AND DEPOSITION 1.1.3. TYPES OF PATTERNS 1.1.4. IDENTIFICATION 1.2. FINGERMARKS 1.2.1. NATURE OF DEPOSITS 1.2.2. NATURE OF SUBSTRATES 1.2.3. DETECTION TECHNIQUES

The organization of microtubules (MTs) in the mitotic spindle apparatus has been shown to involve a combination of centrosomal-based nucleation and hypothesized to involve acentrosomal branching MT nucleation. Here, Petry et al. (2013) uses Xenopus egg extracts to successfully observe branching MT nucleation from existing microtubules utilizing total internal reflection microscopy (TIRF). Direct observation of branching MT nucleation highlighted both the activating effect of RanQ69L and its effector

Patients and methods This is a longitudinal cross sectional study of all children and adolescents patients with LN following inPediatric Allergy and Immunology clinic, Children 's Hospital, Ain Shams University in the period between October 2013 and February 2014. Lupus nephritis in patients with pediatric onset SLE was diagnosed and graded on pathological basis according to 2003 ISN/RPS classification of LN into Class I: Minimal mesangial LN. Class II : Mesangial proliferative LN. Class III: Focal

My research subject is Lyme disease. This is also known as Lyme Borreliosis or just Lyme for short. Lyme disease is an infectious disease of Borreliosis bacteria that is spread to humans via Ticks.  It is a bacterial infection caused by Borreliosis Burgdorferi, a type of bacterium called spirochete. Spirochetes are long, thin spiral shaped bacteria that wiggle and spin to propel through the bloodstream. Once in the circulatory system, Lyme can spread to other parts of the body and cause a variety

Lundgaard et al. (2015) also recognized the conflicting hypotheses in cerebral metabolism and that until this uncertainty is removed, the reliability of neuroimaging data interpretations is reduced. To address this problem, they created a near-infrared 2-deoxyglucose probe (2DG-IR) and used two-photon microscopy to assess whether it is neurons or astrocytes which are taking up more glucose during neuronal activation. If the ANLS hypothesis is correct, astrocytes should be observed taking up more