Immunofluorescence

solution (1% goat serum, 10X PBS, 10% Triton X, DDH20) at 4 C overnight. Nerves were then transferred into primary antibodies in PTX containing 10% goat serum and incubated for 72 hours at 4 C with gentle rocking. Primary antibodies used for Immunofluorescence included mouse monoclonal RT97 (Anti-Neurofilament 200 kDa) (1:5), chicken polyclonal Anti-GAP43 (1:200), and rabbit polyclonal Anti-Fluorogold (1:250). After incubation, nerves were washed with PTX 3 times for 15 minutes each, then washed in

Researchers use double immunofluorescence staining to detect YFP amd TPH. In Figure 2b and 2d there was high number of TPH and YRP for p38αCKOePet and p38αCKOGFAP during Rosa-reporter. While the p38αCKOSERT show less sign of TPH (2c). In p38α expression shown in p38+/+ p38α show more

1 Animal Health Fact Sheet INFECTIOUS REPRODUCTIVE DISEASES OF SMALL RUMINANTS Clell V. Bagley, DVM, Extension Veterinarian Utah State University, Logan UT 84322-5600 June 2001 AH/Sheep/19 INTRODUCTION Several diseases which infect small ruminants result in abortion or reduced fertility and some may also infect humans (zoonotic diseases). Each of the diseases listed below will be briefly outlined. Those marked with an asterisk (*) may also cause human disease. Akabane Disease Bluetongue

The question of this article is to determine if skin dermal papilla (DP) cells can differentiate into induced pluripotent stem cells (iPSCs) by using the transcription factor, OCT4, instead of using all four transcription factor, OCT4, Sox2, Klf4, and c-Myc, which are usually used to differentiate somatic cells into stem cells. The scientists came up with the question because two out of the four transcription factors, Klf4 and c-Myc, are oncogenic gene, thereby it is best to replace these genes

Renal biopsy an invasive procedure in which a sample of renal tissue is obtained(1) . Microscopic examination of the biopsied tissue can provide information needed to diagnose, monitor and/or treat renal disorder. The introduction of renal biopsy in clinical practice has represented one of the most important advances in the field of clinical nephrology. Renal biopsy has contributed greatly to l classification of intrinsic renal diseases and, therefore, to a better knowledge of the pathogenesis involved

There are many tests are available to diagnose SLE. The tests available are antinuclear antibody (ANA), complete blood count (CBC), chest x-ray, serum creatinine, and urinalysis [24]. CBC is used to check red blood cells, white blood cells and platelet level which are typically abnormal in patients with SLE [24]. Urinalysis checks for kidney function, measuring the levels of protein and check for any urinal tract infection [68]. A complete physical is also conducted and the heart is checked for abnormal

Bystander effect in cancer Cancer is defined as unregulated growth of cells that deviate from usual cellular activities (NIH, 2015). This includes the development of tumors and eventually the invasion of other tissues. Metastasis of tumor cell undergoes multiple steps: invasion, intravasation, extravasation, survival and proliferation (Sethi & Kang, 2011; Van Zijl, Krupitza, & Mikulits, 2011). As GJPs have been described to provide a conduit for intracellular communication, it has been shown in

contact their general practitioner if they had flu-like symptoms with facial edema or muscular pain. Case Definition Confirmed case – defined as a person living in Auvergne with either muscle biopsy positive for trichinella or in whom serial immunofluorescence (IF) assays demonstrated seroconversion. Probable case – defined as a patient in Auvergne between February 15 and March 15 1991, with recent clinical features of trichinosis (fever, facial edema or myalgia) or eosinophilia (>1 x 109 eosinophils

Apremilast for Treatment of Recurrent Erythema Multiforme Introduction Recurrent erythema multiforme (EM) is an uncommon skin condition with unclear etiology considered to be a hypersensitivity reaction presenting commonly in young adults with distinctive targetoid skin lesions with or without mucosal lesions. These cases are typically treated with antiviral therapies or immunosuppressive agents in more extreme cases. We present a case of recurrent EM in a young adult that resolved completely after

Lyme disease is also called Lyme borreliosis. It is caused by a bacterium called Borrelia burgdorferi, and transmitted by the black-legged tick, Ixodes scapularis, in the nymph stage (J.F. & Magnarelli, 1993). Therefore, the infected nymph’s density is the most specific ecological indicator of the risk of Lyme disease (J.F. & Magnarelli, 1993). It is an enzootic disease, although humans have been infected as accidental hosts, making it a zoonotic disease (G. & Fish, 1993). The Peromyscus leucopus