In situ hybridization

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  • A Compartment Of Tendon Progenitors

    1266 Words  | 6 Pages

    Upon confirming that axial and intercostal tendons originated from the somites, the researchers wanted to determine if the other somitic compartments affected the syndetome fate. Double whole-mount in situ hybridization was used to compare Scx in progenitor cells, Pax1 in sclerotome, and MyoD in myotome. Scx expression was restricted to the anterior and posterior borders while MyoD expression was present in the center of the myotome with no overlap of the two

  • Myeloproliferative Neoplasms

    977 Words  | 4 Pages

    chromosomal gain or structural rearrangement or at least three cells with the same chromosome deletion. Karyotypes were recorded according to the International System for Human Cytogenetic Nomenclature (ISCN) 2013.14 In addition, fluorescence in situ hybridization (FISH) was performed in most cases (n = 158). The following commercial FISH probes were used in subset of patients: a BCR/ABL dual-color, dual-translocation probe (n = 158); the 13q14 SpectrumOrange probe (n = 66); the LSI D20S108 (20q12) probe

  • Enrichment Imunofluorescence And In Situ Hybridization System In Pancreatic Cancer

    1249 Words  | 5 Pages

    This article is about how to detect circulating tumor cells using negative enrichment immunofluorescence and an in situ hybridization system in pancreatic cancer. Pancreatic cancer is known as the most lethal malignancy with an extremely 5-year survival rate. Circulating tumor cells are the tumor cells that fall off from solid tumor masses and travel into the peripheral blood circulation, and they have been popularly detected by the CellSearch system and used as promising biomarkers to monitor chemotherapeutic

  • Chronic Myeloid Leukaemi Symptoms And Treatment

    795 Words  | 4 Pages

    or BCR-ALB transcript in the peripheral blood or the bone marrow cells (Hehlmann et al.2007). However, if Ph chromosome is not detected conformation can also be done by either reverse transcriptase polymerase chain reaction (PCR) or fluorescence in situ hybridisation (FISH) (Hehlmann et al.2007). Fig 1: Above figure

  • What Is A Cure For HIV-AIDS?

    799 Words  | 4 Pages

    Embretson et al., 1993b, Tamalet et al., 1997). In situ techniques were crucial in demonstrating the importance of the lymphoid follicles in different lymphoid tissues during primary infection in non controllers (Embretson et al., 1993b, Pantaleo et al., 1993) and in elite controllers (Fukazawa et al., 2015). The existence of trapped virions on follicular dendritic cells (FDCs) was first demonstrated by electron-microscopy, then by in-situ hybridization in the 1980s (Armstrong and Horne, 1984) (Biberfeld

  • Methods And Materials : Primer Design And Cloning

    768 Words  | 4 Pages

    Methods and Materials: Primer Design and Cloning: To explore regions of the Drosophila genome that weren’t categorized by Stark lab fragments, we used a molecular approach to design novel candidate enhancers from the ChIP sequence peaks. This approach also allowed us to create narrower fragments (approximately 1000 bp), focusing on enhancer activity and reducing the chance of background activity. Fragments containing in vivo Bcd and Otd binding signals were selected by searching between 500 bp

  • Breast Cancer Essay

    751 Words  | 4 Pages

    (negative), 1+ (also negative), 2+ (borderline), or 3+ (positive- HER2 protein overexpression). The Fluorescence in Situ Hybridization (FISH) test finds out if there are too many copies of the HER2 gene in the cancer cells. The results of the FISH test can be positive (HER2 gene amplification) or negative (no HER2 gene amplification). The Subtraction Probe Technology Chromogenic In Situ Hybridization (SPoT-Light HER2 CISH) test finds out if there are too many copies of the HER2 gene in the cancer cells. The

  • The Mechanisms and Management of DCIS

    1344 Words  | 5 Pages

    programs worldwide lead to significant increase of the rate of detection of DCIS which nowadays represents approximately 20% of all new diagnoses of breast tumours 1-4 Biological characteristics of DCIS and pathways of evolution Ductal carcinoma in situ (DCIS) is defined as an intraductal epithelial cell proliferation with morphologic features of malignancy, but without any evidence of basement membrane penetration. DCIS is lined by a layer of semi-continuous myoepithelial cells and surrounded by

  • How The Staining Of The Centromere Sequences Using Fish

    1927 Words  | 8 Pages

    How the staining of the centromere sequences using FISH can be used to determine the sex of metaphase chromosomes and how immunostaining of the synaptomenal complex of meiotic cells can show the stages involved in prophase. Abstract Fluorescence in situ hybridisation (FISH) and immunostaining are both processes that allow for specific features within a chromosome to be observed. FISH is when a DNA probe that is fluorescently tagged complementary binds to a specific sequence in the chromosome, in this

  • Symptoms And Treatment Of Uni / Bi Lateral Coronal Synostosis Syndrome

    1455 Words  | 6 Pages

    Saethre-Chotzen syndrome is a craniosynostosis which the main clinical feature is the presence of uni/bi-lateral coronal synostosis. Other facial clinical features are strabism, bulgy eyes and small sized ears with a peculiar prominent cus. Patients can also present syndactily of digits with various severities. Mutations in Twist1 are the only responsible for this specific syndrome. The mutations so far observed lead to the production of truncated or non-functional TWIST protein. Saethre-Chotzen