In situ hybridization

For example, when I was present, a patient’s bone marrow aspirate smear was being examined by one of the haematology registrars. Once the examination was complete, the Registrar asked a Cytogeneticist about the results of their Fluorescence in situ Hybridization (FISH) regarding the same patient. MDT’s reduce the isolation between different departments, allowing members of the team to express their opinions. This allows the team to form more unified decisions regarding a patient’s treatment or diagnosis

chromosomes can also be analyzed and they can find the missing or misplaced chromosome. In this case they would find that the fifth chromosome is missing. They can also do a more detailed test called the “FISH” test. FISH stands for Fluorescent in Situ Hybridization, it can detect the deletion. Who has this disorder: A commonly asked question for all genetic disorders is “Who get’s it”? The answer is simple. Anyone can be born with 5p syndrome. 5p syndrome is not more prominent in any group of people

Planarians are members of the Platyhelminthes (flatworm) family and are triploblastic organisms whose tissues arose from the three germ layers namely; ecto-, meso- and endoderm. They act as a good model for cell proliferation and therefore regeneration. Planarians are composed of many organ systems including a centralized nervous system (CNS) which is made up of bi-lobed cephalic ganglia that are positioned at the anterior end of the body. These ganglia are linked to two longitudinal ventral nerve

My work in the lab produced several tangible products consisting primarily of collected samples. Throughout my work term I collected several samples from secrtogranin II (SgII) knock out (KO) fish. Because the SgII gene possesses two paralogues these fish could either be SgIIa KO, SgIIb KO or SgIIa/b KO. Samples from wild type (WT) fish were also collected with the KO samples in order to see any consequences of knocking out SgII. A number of the samples from the KO animals were used for RNA extractions

Adam Murphy Dr. Thornton Genetics 10 October 2014 Reproductive Genetics In a post-genome age, reproductive genetics is becoming an increasingly pressing topic in the debate between advancements in genetic research and ethical policy. Today, parents are given an unprecedented amount of control over the future health of their children. Many different methods of genetic screening and prenatal testings are becoming more and more available to the public; however, due to the nuance

In every cell in the body there is a nucleus and this is the location where genetic material lives. These genes are responsible for carrying the inherited traits that we get from our parents, in the form of chromosomes. Normally each cell contains a nucleus with a pair of 23 chromosomes, half of which come from mom and the other half coming from dad. The genotype in the example given is that of a person with Down syndrome. Down syndrome is a chromosomal disorder that results in learning disabilities

DNA pattern, missing chromosome, or gene mutation. Parental DNA pattern test screens for three of the four genetic defaults that cause this syndrome. To find out if the chromosome is missing either a fluorescence in situ hybridization (FISH) test or a comparative genomic hybridization test (CGH) can show if portions of chromosomes are missing. It is rare if Angelman syndrome occurs with only a gene mutation. This happens when the UBE3A gene is present and active, but mutated. A UBE3A gene sequencing

Kosuri, 2012). Other scholars, such as Xiao, Lu, Qin, & Lai, (2006) reported that the use of DNA storage facilitates the use of DNA as information carrier and the modern biological technology as the main implementation tool that makes sequence hybridization and other techniques of isolation, synthetization, amplification, digestion and sequencing of DNA very easy. Other researcher, such as, Glenn (2011), indicated that this technology gave researchers like himself tools that are opening new avenues

resistant to treatment procedures. Not only does CCND1 amplification preferentially occurs in ER positive breast cancer patients, but also eight different studies have shown data linking poor response of tamoxifen therapy too CCND1. FISH (Fluorescence in situ

Breast cancer is the most common cancer in women in the world. Though the unknown nature of breast cancer etiology is still on debate, there are numerous risk factors that influence the development of the disease including genetic, environmental, hormonal, sociobiological and physiological influences. The impact of breast cancer is deep in women diagnosed with the disease and their families with fear and anxiety over the eventual outcome of the disease manifesting itself through behavioral changes