Luciferase

Transgenic Mice, Care and Handling Double transgenic mice express a chimeric mouse/human amyloid precursor protein (Mo/HuAPP695swe) and a mutant human presenilin 1 (PS1-dE9) were purchased from The Jackson Laboratory (004462, Bar Harbor, ME, USA). All animal treatments were approved by the Animal Ethics Committee of Tsinghua University. Mice had access to pre-filtered sterile water and standard mouse chow and were housed under a reversed day-night rhythm in accordance to local legislation on animal

Bioluminescence is a chemical reaction giving off light that emits from a living thing. Unlike other light, this form of light is not hot to the touch. Biofluorescence is a different way of giving off light from other animals. Fluorescence is not a chemical reaction, and happens when exposed to another source of light. Both of these natural ways of emitting light are found in many animals, but need specific components to work. Eighty percent of all living things known to produce light Iive in

Results MiR-365b-3p regulates proliferation, migration, invasion and apoptosis of human lung cancer cells In order to investigate the expression of miR-365b-3p in normal cells and lung cancer cells, we used real-time PCR to measure the expression of miR-365b-3p in various cells. Results showed that the expression of miR-365b-3p was significantly lower in lung cancer cells compared with that in normal cells (Figure 1). After transfection, the expression of miR-365b-3p in COLO 668 cell line was successfully

BIOC 3014 Coursework Kai et al IkB Kinase β (IKBKB) mutations in lymphomas that constitutively activate canonical nuclear factor kB (NFkB) signalling. 2014, J Biol Chem 289(38)26960-72. http://www.ncbi.nlm.nih.gov/pubmed/?term=J+Biol+Chem+289(38)26960-72 Each question is marked out of 25%. The technique and detail parameter was subtracted from the paper directly used as a instruction and reference. 1. Discuss in detail the effects that an activating mutation in the IKBKB gene would be expected

using MTT assay. Cell invasion and migration were evaluated using Transwell assay chambers. Prediction of a regulatory relationship between microRNA-22-3p and 3'-UTR of TP63 mRNA was performed by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. OC cell lines Lung cancer cell lines H292, PC-9, CL1-5, A549 and normal NHBE cells were used in this study. All of Lung cancer and normaL ceLL Lines - were provided by ATCC. H292, PC-9, CL1-5, A549 and NHBE cells were cultured in

Hops, as an Alternative Botanical Treatment Option and Dietary Supplement? Hops are most commonly associated with beer, however, hops have a long history of different uses in the field of herbal medicines and botanical treatment. With the different uses of hop extracts such as, xanthohumol, isoxanthohumol and 8-Prenylnaringenin, could hops pose as a new botanical treatment option for many medical issues and diseases? As Martorana, (2010) explains, the first evidence of hop cultivation showed up

Another key study, performed by Darlington et al, analyzed the function of dCLOCK, the CLOCK protein in Drosophila. This study confirmed the identity of dclock, investigated the function of dCLOCK in regulation of the circadian genes per and tim, and demonstrated the function of PER and TIM in an inhibitory feedback loop (Darlington et al., 1998). First, a screen for a homolog of the mouse clock gene in Drosophila was conducted using the cDNA library of adult Drosophila heads and a probe of the mClock

knock-out embryos at different stages. We will also examine how the Notch pathway, which plays essential roles in balancing proliferation and differentiation in retinal development, is affected in the mutant retinas by in situ hybridization and in vitro luciferase reporter assays. Aim 3. To investigate the mechanisms by which Zfp36l1 and Zfp36l2 function retinal development. To understand the genetic mechanisms underlying the function of Zfp36l1 and Zfp36l2 in retina development, we will perform RNA-seq

bacterium that served as the model organism in this experiment. We isolated only the luxAB portion of the entire lux operon and inserted it into the pGEM vector plasmid to transcribe the luciferase enzyme required for bioluminescence. Within the lux operon, luxAB is responsible for producing subunits that form luciferase, which oxidizes the aldehyde made by luxCDE into the reduced flavin-mononucleotide FMNH2 and results in the production of light known as bioluminescence[6]. The luxI gene that is also

in basal medium for 24 h, nuclear extracts were prepared and protein concentrations were determined using the BCA Protein Assay kit (Pierce, Rockford, IL). PPARδ Reporter Assay — To determine the activation of PPARδ by 15d-PGJ2 a PPARδ specific luciferase reporter assay was performed using PPARδ Reporter Assay System (IB00121; Indigo Biosciences, PA)