Lysogeny broth

Finding the Minimal Inhibitory Concentration: The Process and the Importance Melissa Bañaga San José State University Abstract The purpose of this project was to determine the minimal inhibitory concentration of our given antibiotic called tetracycline. Tetracycline is a bacteriostatic agent and instead of directly killing the bacteria, it prevents the growth mainly by inhibiting the synthesis of proteins. We hypothesized that the minimal inhibitory concentration for our test would

Assignment 1 for MCB 3020L Spring 2014 (due 2/24 or 2/26) Instructions: * Your answers have to be typed and double spaced. * Put the date, your name and your TA’s name on the cover page of this assignment. * “Give a detailed explanation” means don’t just give a one sentence answer. * Don’t forget to include your calculations and references!  Late assignments are not accepted for grading. Questions: 1) Assume that you measure the OD of two E.coli cultures:

6. Incubate both microcentrifuge tubes on ice for fifteen minutes. 7. Take both tubes out of ice and immediately place in incubator at 42٥C for 90 seconds. 8. After place both tubes back in the ice for two minutes. 9. Add 200uL Luria Recovery Broth to both microcentrifuge tubes. 10. Let both the tubes rest at room temperature for 10 minutes. 11. During the 10 minutes, get the LB agar and LB+AMP agar plates ready. Mark your plates with the transformation tube mixture to use (+ or -), the lab

agent influences the transcription of the GFP and activates the transcriptional promoters. A promoter allows a foreign gene to fall into place much easier resulting in gene expression. It is expected that the combination of the plasmid, with the Lysogeny Broth (LB) and the arabinose (ARB) will transform the bacteria, and allow for growth of the bacteria in the presence of the antibiotic ampicillin (AMP) along with an observable glow under ultraviolet (UV) light. Hypothesis: • The Agar plate with the

First, 500μl of calcium chloride was added to a DNA tube. Calcium chloride makes it easier for cells to transform. Approximately 15 E. coli colonies were then transferred to the tube. 250μl of the DNA was transferred into another tube, where 10μl of pGFP was then added. The plasmid for green fluorescent protein is what will be transformed into the E. coli’s DNA so it will express the GFP. The DNA tubes were then incubated in hot and cold water .This process is called “heat shocking” and affects

The medium LB Agar was used. Fisher Scientific ® Lysogeny Broth or “LB Agar” was created by suspending forty grams per one liter of purified water. Next, the LB Agar was mixed and heated using a magnetic mouse and a Corning ® Stirrer/Hotplate until the solution was transparent and beginning to bubble towards

Table of Contents 1.0 Abstract 2 2.0 Introduction 2 3.0 Aims 3 4.0 Theory 3 5.0 Apparatus 5 6.0 Procedure 5 7.0 Result 7 8.0 Calculation 10 9.0 Discussion 11 10.0 Conclusion 13 11.0 Recommendation 13 12.0 References 13 13.0 Appendix 14 1.0 Abstract In this experiment, Escherichia coli is used as a sample to study the growth kinetic of microorganism in shake flask. A different volume of E.coli was transferred into 250ml Erlenmeyer/shake flask containing media for the nutrient of microorganism

Table of Contents 1.0 Abstract 2 2.0 Introduction 2 3.0 Aims 3 4.0 Theory 3 5.0 Apparatus 5 6.0 Procedure 5 7.0 Result 7 8.0 Calculation 10 9.0 Discussion 11 10.0 Conclusion 13 11.0 Recommendation 13 12.0 References 13 13.0 Appendix 14 1.0 Abstract In this experiment, Escherichia coli is used as a sample to study the growth kinetic of microorganism in shake flask. A different volume of E.coli was transferred into 250ml Erlenmeyer/shake flask containing media for the

The creation of synthetic life forms: the science, benefits, risks and ethics. The creation of a synthetic life form is the production of an artificial autonomous self-replicating organism under in-vitro conditions from biochemicals. Synthetic life experiments and creation of synthetic cells gives us an unparalleled insight into the origin of life, the fundamental genes and essential genetic functions required for life and may someday allow the manipulation of the roles of cells and organisms to

ComGen Labs: Fighting Take-all By Artem Yashchenko Biology 211 Winter 2015 Seattle Central College Lab Partners: Dianna Nguyen Courtney Matzke Madhuri Popuri Abstract The purpose of our lab was to sequence part of the Pseudomonas fluorescens genome trying to find genes coding for the polyketide antibiotic which is thought to be the key component of biological control over Take-all disease. We studied clone #2 and found 100% similarity with genome of Pseudomonas brassicarium