Microtiter plate

The plate was then read for absorbance at 540nm using the microplate reader. The neutral red assay was chosen because it assesses the lysosomal integrity of cells. As the integrity of the lysosome in cells is dependent upon a very wide range of healthy internal

determining the protein concentration of the two given unknown proteins in the sample solutions. The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed in this Assay. Methods: (1) There are two methods using which the BCA Protein Assay can be performed- Test tube method and Micro plate method. The following experiment has been performed using the Microplate procedure. Vial

Question: Would the GFP gene in a pGLO transformed E. coli be activated by a sugar other than arabinose? Introduction: Green Fluorescent Protein, produced by the bioluminescent jellyfish Aequorea victoria, is a protein that fluoresces green under ultraviolet light. Since its discovery, properties of the protein have been improved by mutations in the gene resulting in the expansion of its spectrum, which now contains brighter variants and multiple different colors. GFP is used in a wide variety

Humans have used animals for food, transport, companionship and experimental research to develop medicine and investigate the structure and function of the body. This dates back to 130 AD, where the commonly used animals were pigs, monkeys, and dogs (Vaumans, 2005). Dogs are the species most frequently used in the animal models and are referred to as “default non-rodent” (Smith et al., 2002). Especially, in toxicology studies, dogs are the preferred choice due to their similarities of human toxicity

Arg-and Asn-sensing membranes at the two emission wavelengths (λem = 565 and 625 nm) at an excitation wavelength of 460 nm (λex = 460 nm). The fluorescence spectra for detecting L-Arg and L-Asn were measured using a multifunctional fluorescence microtiter plate reader (Saphire2, Tecan GmbH, Wien, Austria). The Arg-sensing membranes immobilized with various amounts of arginase and 20 U of urease were used for the measurements of different L-Arg concentrations. The arginase amount for immobilization was

"Host Service" because the host plays an active role in the service. Food is brought on platters by the waiter and is shown to the host for approval. The waiter then places the platters on the tables. The host either portions the food into the guest plates directly or portions the food and allows the waiter to serve. For replenishment of guest food the waiter may then take the dishes around for guests to help themselves or be served by the waiter. French Services: It is a very personalized service

1. Introduction The Food and Beverage service wraps the knowledge, technique and skills essential for those studying and working at different levels in the food and beverage sector. The operations in this department are constantly improving and developing over the years together with progress in the quality as per the ever changing customer needs and industry demands. The long established perspective of food and beverage service was considered as just a delivery process with customer being recognized

As soon I took the last bite the waitress appeared right beside me holding in her hands my next dish; Ravioli stuffed with spinach. I couldn’t believe how perfect the timing was. It was something I’ve never witnessed before. The plate of ravioli comes with just the right amount of pieces of an odd number of five. After eating four and a half my stomach was already full from both of the splendid dishes. Immediately after setting down my fork and knife the waitress appeared making

Investigating Plate Margins Plate margins are the edges of the plates, where all the power of nature is released in earthquakes and volcanoes. There are three main types of plate margins: - Spreading Boundaries There are two types of spreading boundary Ridges, where new rock is formed, pushing the plates apart, and Rift, where the plates pull apart, and the earth between them sinks. On the ocean floor the plates are spreading. All along the mid ocean

INTRODUCTION: Tectonic geomorphology is defined as the study of landforms produced by tectonic processes, or the application of geomorphic principles to the solution of tectonic problems (Keller, E.A. and Pinter N. 1996). Geological structure plays a crucial role in determining a wide field of relief forms, even in areas where exogenetic factors are regarded as the dominant ones in the landscape (Ahnert 1998; Bloom 1998; Ritter et al. 2002). In recent years Digital Elevation Model (DEM) data and