Paper chromatography

In this experiment, the process of chromatography was taken place in order to identify the different types of pigments present in spinach leaf. This was done by carrying out two types of experiments which are thin layer chromatography (TLC) and paper chromatography. Both of the experiments were done using a similar procedure except that they both used a different stationary phase. Paper chromatography used paper, whilst, TLC used a silica plate. Propanone was used as the extraction solvent for spinach

experiment performed, which separated carbohydrates in a very similar method with the use of paper chromatography (Inome, Y., & Yamamoto, A.). Proper pipetting technique, which is described by John Husler, was also demonstrated in this experiment. The technique was followed as to prevent contamination and deliver the right amount of solution each time (John Husler: 1983). The use of paper chromatography to separate plant pigments from spinach leaves worked very well. The pigments were separated

that were obtained through the use of paper chromatography resulted in four distinct colors. The very goal of this study was to determine what pigments are present and what there absorbance is through the use of paper chromatography and a spectrophotometer. These colors in turn related to pigments that were recognized as chlorophyll a, chlorophyll b, carotene, and xanthophyll. As for the results related to the observations found within the paper chromatography, these four pigments had a distinct relational

to one another? Procedure: Place a small amount of solvent in separate beakers. Then place the mixture on chromatography paper and put the paper in each beaker. Controls: The chromatography paper used for each of the beakers would have to be the same because different chromatography papers may contain different amounts of cellulose, which would vary how attracted the solvent is to the paper. The amount of time allowed for the dyes to travel also has to be the same for each solvent in order to obtain

creating colored candies such as paper chromatography. For instance, if one wants to know how M&Ms get their different colored shells would it be valid to use paper chromatography to come up with a reasonable solution? The purpose of this experiment is to physically separate the different dyes that are used to create the variety of M&M colors while using paper chromatography. Lastly, the experiment results were satisfactory, but were not 100% accurate. Paper chromatography does indeed separate mixtures

The objective of this “Chromatography Lab” is to determine the number of substances and their Rf values present as dyes in different markers/inks. Chromatography deals with physically separating a mixture into it’s very own components. Chromatography is used for keeping compounds from mixtures. The process that is involved in it, is separating out a mixture of chemicals, that are most likely either in a liquid or gas form. This process allows the chemicals/substances, to past each other, which is

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences. Chromatography works on the basis that different molecules have different polarities. By allowing molecules to travel through a polar surface, it is not surprising that molecules will different polarities will travel a different amount. The substance, or stationary phase

In this lab, paper chromatography will be used to separate the components of known and unknown mixtures and then used to identify those components. Paper chromatography is a technique where a drop of solution containing a mixture will be placed on a piece of filter paper. One end of the filter paper will then be placed into a liquid solvent. The mixture will separate into its different components as the solvent moves up the filter paper. The filter paper is known as the stationary phase. The solvent

different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll

Paper Chromatography and Spectrophotometry Used to Identify Amino Acids and Concentration of Protein in Solution Introduction: Paper chromatography was used to characterize known amino acids in solution with an accordant Rf value. These Rf values were then used to identify a solution with unknown amino acids and observed Rf values. An Rf value is calculated using the following formula:Distance Traveled by solute (cm)Distance Traveled by solvent (cm), where the distance traveled by solute is measured