Paper chromatography

Thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In 5.301, and in most lab applications, you will use standard phase silica plates. You will apply your reaction mixture

of the fractions produced is redistilled to increase the purity of the liquid. Since two rounds of distillation occur, the process is termed fractional distillation. Additionally, it is crucial for students to understand the principles of gas chromatography so the purity and percent composition of each fraction can be determined. Theory: The basis for this

experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a, chlorophyll b, carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light. These results along with previous research

with the chromatography strip positioned 152 mm tall and 19 mm wide. Using a ruler and pencil, we drew 15 mm from the bottom of the paper across the width. We measured 9.5 mm from the edge and placed a dot with the pencil on the line to mark the starting point. The, we measured 20 mm from the top of the strip and folded across the width of the strip. These few steps were repeated with a second strip. Next, the red and purple dyes were obtained. Using 2 clean toothpicks, the chromatography strips were

Pyrolysis is the decomposition of organic matter by utilizing heat. Pyrolysis is a chemical test, meaning particles go through a process of being dried by increasing heat to extremely high temperatures which finalize the result for identification or specific findings. Pyrolysis aids in analyzing paint, but other components can be determined through this process. Ultimately, the concept of pyrolysis is that it can be broken down into multiple chemical properties, which also means that the advantage

During the first part of the experiment a dot of ink was placed onto chromatography paper to separate the mixture by pigment. This chromatography paper was place so that a portion of the paper below the dot was in water, so that the water could travel up the paper and separate the ink. The paper was suspended using a toothpick that was put through the top of the paper, which was held by the neck of the bottle. The second part of the experiment dealt with the separation of a mixture of iron, sand

Exploring Thin Layer Chromatography Thin layer chromatography (TLC) is used to to identify organic compounds with common over-the-counter medicine; and the relative strength of the intermolecular forces and how they affect the compound’s polarity within the medications tested. TLC is the physical separation of a mixture into its individual components by distributing the components between a stationary phase, or silica gel; and a mobile phase, or movement of sample up the TLC plate to the solvent

Draft 5 Analytical Differentiation of 1-Alkyl-3-acylindoles and 1-Acyl-3-alkylindoles: Isomeric Synthetic Cannabinoids Jack DeRuiter, Forrest T. Smith, Karim Abdel-Hay and C. Randall Clark* Department of Drug Discovery and Development Harrison School of Pharmacy Auburn University Auburn, AL 36849 * Corresponding Author: Professor C. Randall Clark, Ph. D. clarkcr@auburn.edu phone 334-844-8326 fax 334-844-8331 Abstract The 1-alkyl-3-acylindoles and the inverse regioisomeric 1-acyl-3-alkylindoles

plants. Abby was certain that the green section contained chlorophyll, whereas, Estelle claimed that both the green and purple parts of the coleus plants consisted of chlorophyll. A paper chromatography test was conducted to clearly separate the mixture of the pigments in which the mixture was dropped onto a slip of paper and the end was then placed into a solvent. After analyzing both the green and purple sections of the leaves, our data affirmed Estelle’s statement who claimed that chlorophyll existed

TA points 0 FINAL TOTAL POINTS 71 INTRODUCTION In the first part of this experiment, an ester product will be isolated and purified from unknown alcohol. Then the product ester will be identified through analysis of boiling point, gas chromatography, and IR spectroscopy. In the second part of the experiment, Biodiesel will be synthesized from vegetable oil by transesterification. DATA AND CALCULATIONS FISCHER ESTERIFICATION UNKNOWN ID: UNKNOWN NUMBER: ___F________________________