Paper chromatography

Summarized Results To construct a calibration curve, five standard caffeine solutions are prepared and their retention times and peak areas are obtained from the High Performance Liquid Chromatography (HPLC) (Table 1). A graph of peak area versus concentration of caffeine standard solution is plotted (Figure 1). The slope of best fit line and intercept of the calibration curve are 97160384 and -3134906.6 (equation 1 and 2). The standard deviation of slope is 45811392.43 (equation 3). The standard

pigmented compounds within MI-1 and MI-2. These methods were column chromatography with a silica base gel and solid phase extraction utilizing LC-Diol and LC-Si cartridges. These methods all revolve around trying to purify polar compounds from nonpolar impurities and were chosen since prodigiosin is a polar compound containing three pyrrole rings (6). Upon completion of techniques, two primary compounds were extracted via column chromatography: a red pigmented compound, and a UV sensitive compound. The UV

In this lab a cotton swab was fluffed up by scraping it against the serrated edge of a tape dispenser and then dipped into a liquid chemical solution. Once dipped the cotton swab was placed horizontally above the fire making sure to only burn the cotton part so as to not accidently get a different flame because of the burning stick. The color of the flame is viewed through a spectroscope and then both that spectrum and the color to the naked eye are recorded on a data table. This step is repeated

The unknown substances found using both methods of chromatography could be identified using the calculated retention factor (Rf) values. The equation is below: The Rf values for the substances on the paper chromatograms were calculated using this equation as follows: The Rf values for the substances on the thin-layer chromatograms were also calculated as follows: Below is a table containing the colour and retention factors of different substances found in plants: The retention factor of a compound

investigating samples of polluted ground water will want to know which toxic ions might be present in a sample. Chromatography is one of the first tools used in such situations. In this technique, many types of mixtures can be separated into the component pure substances; by comparison to a standard sample, each component substance can also be tentatively identified. Many varieties of chromatography exist, each one designed to separate specific types of mixtures. The common feature of each type of

21. 1912, Picasso, Pablo, Maquette for Guitar, Paris, Spanish, Cubism. TXT- Picasso constructed this piece using cardboard, string, and wire, which was bases from his distinguished work of Guitar, Sheet Music, and Wine. This specific sculpture was used to express the interior aspects of the guitar to view the volumetric attributes and ideas behind the creation itself. Page 156 CLS- The abstract qualities depicts the interior of the instrument to show what’s inside and the purpose of each attribute

In this experiment, thin layer chromatography (TLC) was used to identify and compare polarity of two molecules, caffeine and acetaminophen. Chromatography is defined as the separation of a mixture of chemicals as they flow at different rates over a stationary phase based on their relative polarity. Caffeine, the more polar molecule had a greater affinity for the polar silica gel stationary phase causing it to consistently have a lower retention factor regardless of the mobile phase. This methodology

two-step reaction, 1-phenylethanol was reacted with vinyl acetate with the help of acylase I to form an ester, unreacted 1-phenylethanol, and vinyl alcohol. The unreacted 1phenylethanol was separated from the ester by column chromatography and confirmed by thin-layer chromatography (TLC). In the second

dilutions of PNP are prepared, and the Bradford Method was used to measure protein concentration. There are different types of chromatography, and we would be using gel filtration and affinity. Chromatography is defined as the separation of molecules based on properties such as molecular mass, charges or solubility. In Experiment 5.1, gel filtration chromatography was used to separate molecules based on their molecular mass. The stationary phase in the column contains tiny pores, those that are

In this experiment, separation of fluorene and 9-fluorenone took place using column chromatography. The pureness of the separate compounds was checked using TLC and melting point analysis. Rf values were the determined from the TLC plates and calculated to compare the experimental Rf values to the starting mixture Rf values. TLC is useful to separate tiny samples and is considerably quick, while chromatography is used to separate considerably larger mixtures but takes more time. The melting point