Peptide bond

Amanda Sledge Dr.Draughon Bio 111 14 February 2016 Testing for Peptide Bonds in Solutions & Water using Biuret Reagent Abstract This experiment was performed to test an albumin solution, gelatin solution, glucose solution, water and an unknown solution for the presence of peptide bonds resulting in proteins through the use of biuret solution. 5mL of each solution was distributed individually to an assigned test tube to which the biuret solution was added. Ultimately the albumin and gelatin

Q 1.     In this practical exercise you will be using biuret reagent.  Why is biuret reagent used in this practical? The biuret reagent is used to assess the concentration of the protein because peptide bonds occur with the same frequency per amino acid in the peptide. The intensity of the colour, and therefore the absorption, is directly proportional to the protein concentration. Q 2.     What is the experimental reason for constructing a standard curve? Multiple samples with known properties

Mannose-binding lectin is an important component of the innate immune system. MBL is originally synthesized in the liver, circulates throughout the body and has the ability to identify a broad range of pathogens based on recognition of carbohydrate repeats displayed on microbial surfaces . 3.4.1 Discovery of mannose binding lectin In 1968, a case of an infant with plasma-dependant defect in phagocytosis of yeast particles was described. The cause was related to a deficiency in a plasma factor rather

Nitrogen is used by plants in order to synthesize protein peptide bonds and for cell growth. Not only is this nutrient required in the largest quantity by plants, but it is also the most frequently limiting factor when it comes to productivity in crops. Plants cannot use nitrogen in the air and in the soil system it is lost easily. Because of this plants are forced to obtain nitrogen in the form of nitrate and ammonium from the soil. Too much nitrate can cause a negative effect on the plant including

The Chemistry of Enhanced Sports Performance Other than being athletes, Tyson Gay, Marion Jones, and Steve Mullings all have something in common. They have all been caught for using performance-enhancing drugs which was also known as doping. The concept of doping exists because athletes traditions to achieve and perform well can conflict with standards of conduct requiring these impressive results be achieved in an ethical manner (Hoberman 2016). Just like the well-known saying “practice makes perfect”

Moreno Diego Gene Cards Assignment 09-26-17 Gene: BAP1 1. What type of mutation it is (loss of function, gain of function—look up ANY terms up in your book if you don’t know what they mean! The BAP1 gene belongs to the ubiquitin hydrolase subfamily of deubiquitinating enzymes. It is a recessive mutant gene. 2. Does it act in a dominant or recessive manner? Bap1 acts as consistent recessive cancer susceptibility gene. 3. What is the affect of the mutation on the cell cycle? BAP1 is a recessive

In order to improve photolytic deprotection with enhanced efficeincy, several other photoremovable groups have been developed. Figure X.X.X shows o-nitrobenzyl protecting group together with other common photolabile protecting groups such as 1-(o-nitrophenyl)ethyl (NPE), 2-(o-nitrophenyl)-propyl (NPP), 6-nitroveratryloxycarbonyl (NVOC), 2,2-bis(2-nitrophenyl)ethoxycarbonyl (diNPEOC), 4-N-[2-(2-nitropehnyl)-propoxy]carbonyl (NPPOC), 1-(3-nitrodibenzofuran-1-yl)ethyl (NDBF), 6-nitropiperonyloxymethyl

amino acids to replace the naturally occurring alpha equivalent has recently allowed scientist to explore a more stable peptide mimic. The ability of beta-peptides to resist protease degradation has vastly improved their ability to be drug candidates as well as other therapeutic targets. As a result, it will be explored in this paper whether or not peptide mimics with beta peptide backbones are able to function as transporting polymers for short interfering RNA (siRNA). siRNA has the ability to silence

In general, the gap is broadening rapidly between the number of known protein sequences and the number of known protein structural classes. To overcome this crisis, it is essential to develop a computational prediction method for fast and precisely determining the protein structural class. Based on the predicted secondary structure information, the protein structural classes are predicted. To evaluate the performance of the proposed algorithm with the existing algorithms, four datasets, namely 25PDB

3. Results 3.1 cDNA cloning and sequence analysis In this study, we cloned the rabbit NMU, NUM-R1 and NMU-R2 cDNA fragments using RT-PCR. Specific fragments were amplified and target gene fragment length was 443, 366 and 203 bp, respectively (Fig.1 A, B and C), consistent with the expected size of the fragment, and clear bands. These fragments were confirmed by sequencing, and the NMU, NMU-R1 and NMU-R2 sequences were submitted to GenBank (GenBank IDs: KP276160.1, KM-236787.1 and KM-236788.1). NCBI