Phantom Blot

Nematodes were cultured and handled at 20 °C using standard methods (Brenner, 1974). C. elegans strains used in this study are as follows: N2 Bristol and MR156: rrIs01[Pelt-2::GFP; unc-119(+)] X, which were used as wild type. CB3514: lin-23(e1883)/dpy-10(e128) II, YHS47: lin-23(e1883)/dpy-10(e128) II; rrIs01[Pelt-2::GFP + unc-119(+)] X, DH2: zyg-11(b2) II. DS97: mat-2(ax76) II, EU593: mel-26(or184) I, EU640: cul-2(or209) III, UP63: mat-3(ku233) III, NJ582: cul-1(e1756)/unc-69(e587) III, VC141: zif-1(gk117)

Proteomics is the study of protein and how they interact and function within organisms. This results in catalyzing and cataloguing proteins; while comparing how each variation of protein expression in different conditions. This is done in a large scale manner; a good example of this is the Human Genome Project that took place from 1990 to 2003. With the field of Proteomics it has given the ability to understand genes and to comprehend why there’s so much diversity of protein, including their importance

Immunoblotting is an effective approach that utilizes antibody-tags to analyze, isolate, and quantify proteins. A Western Blot, a type of immunoblotting method, was used in this experiment to evaluate the protein quantities contained within the different subcellular fractions. Although, the Western blotting method can be implemented for the study of more complex cellular processes. Complex cellular processes such as metabolism can be greatly evaluated with immunoblotting methods. For example, some

Imagine a world where an innocent child is given HIV due to mother to child transmission. Imagine a world where a father cannot play football on an autumn’s afternoon with his son because he constantly feels a lack of energy. Imagine a world where for months straight one has a reoccurring yeast infection. And lastly, imagine a world where one cannot remember one’s very own mother’s name due to short term memory loss. Unfortunately, there is no need to imagine for all of the above are actual possibilities

Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease

1) I would like to become an HIV Peer Test Counselor because I want to empower students with the knowledge and agency to live healthy lives as they balance their sexual, emotional and physical health. Taking an HIV test is an intimidating experience for many people, so I want to provide my peers a warm, anonymous, and safe space where they can share their experiences while I help them make informed decisions that reflect their cherished values and beliefs. This role will help me increase my emotional

The mobile clinic will be operated for providing HIV testing and counseling service. Weekly outreach events will be hosted on a total of 5 college campuses in Alameda County at UC Berkeley, Cal State Hayward (East Bay), Chabot College, Laney College, and Mills College. The outreach on the campuses provides awareness and educate college students regarding the HIV cause, transmission, and prevention. The intervention proceeds over a 24-month period with participants between the ages of 20-29 years

Abstract Forty million people worldwide are infected with the HIV virus. About six percent of them will not inform their intimate partners about their health condition. Many efforts that have been made over the past decade towards establishing a HIV/AIDS law, have finally paid off. The act of disclosing the virus was written in 1990. It caused quite a stir among the citizens of the United States. Many people concluded that there were holes in the disclosure law concerning HIV/AIDS because it lacked

Mandatory HIV testing should be enforced, because for those who have the virus and do not know that they have it, could possibly be spreading it to many of partners. If the government starts enforcing this potential law, it would prevent many misfortunes and burdens. An article read, “It will help people make informed choices. Some people are not honest enough to disclose their HIV status” (Obemeyer). This quote explains that for those who do not have HIV, will not make informed choices about intercourse

Western Blotting. Western blotting is the transfer of proteins from a gel to a membrane for subsequent analysis. This method can produce qualitative and semi-quantitative data regarding the desired protein. Western blotting requires multiple steps involving gel electrophoresis for separation of macromolecules, followed by blotting onto a matrix, selectively probed, and lastly detected using a technique dependent on the desired protein and experimental design.9 Kinase Assays. Kinase assays measure