Pigments

Absorption, and Effectiveness of the Driving Forces Behind Photosynthesis: Pigments Kathryn Sawyer Abstract To understand pigments and their part in the essential process of photosynthesis, we constructed an experiment to discover first-hand the effectiveness of specific pigments found in pimento leaves. These two exercises would specifically focus on the difference in polarities and the different wavelengths at which each pigment absorbs light. The ultimate source of energy for most organisms is sunlight

of California-Davis, 2013). In the experiment conducted, paper was used as the stationary phase, and chromatography solvent was used as the mobile phase. With the help of chromatography paper and chromatography, three types of pigments were found in the plant.

Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it

the water (H2O) comes from what the tree is able to absorb from the soil. The light in the equation comes from the sun. This is able to be absorbed by different pigments in the plants leaf. The pigments are stored in organelles called chloroplasts; these organelles are what is responsible for photosynthesis. There are may different pigments in each leaf. In this experiment we determine what wavelength of light is optimum for energy absorption in a spinach leaf. I believe that the violet light will

mixture. The crude pigment was sent through the column however three distinctly separate layers were collected (yellow, green-blue and blue). Each coloured layer represented a different pigment. It was made clear by the column chromatography that the beta-carotene (yellow) is the least polar pigment, chlorophyll b (green) is the most polar and chlorophyll a (blue-green) is intermediately polar based on the order the pigments were eluted. In the column, the least polar pigment eluted first as it adhered

The purpose of this experiment was to learn about the analytical method known as chromatography which allows the separation of a mixture into its molecular components. In order to illustrate this technique the pigments found in the leaves of spinach were separated by means of thin layer chromatography (TLC). All chromatographic techniques have one principle in common; a liquid or gaseous solution of sample, called the moving phase, is passed (moved) through an adsorbent, called a stationary phase

primary focus of this experiment. We did a couple different tests in order to find out how the pigments in plants make photosynthesis possible. Paper chromatography was carried out in order to find out the solubility and polarity of each pigment we tested. Also each pigment was put in spectrophotometer to measure the absorption and wavelengths. Out of the four pigments we tested, our accessory pigment β- Carotene proved to have the greatest absorption and polarity. Introduction Photosynthesis

experiment is to classify the pigments in various plant specimens as one of the four main pigments by recognizing distinct characteristics indicative of each pigment. If the water keeps the color of the specimen, then I will be able to identify which pigment (or perhaps pigments) is present in the specimen. I came to this conclusion based on the logic that some of the four pigments are water-soluble, so if the water has no color, the pigment dissolved and I can eliminate said pigment. I think that my ability

Pigmentation The concepts of pigments in metabolism are applicable to this condition and are important in societal understanding. Pigments can be found in leaves, medicine, and food. Pigments contain chlorophyll a and b depending on the type of plant, where chorin wavelengths of both colors of blue and yellow are shown in the white light. This color gives the plant a green pigment. Plants reflecting green pigments are when the bouncing through the accessory pigments, going to the antenna complex

provided table): Objective: (3 mark)* What is the purpose of this experiment? The purpose of this experiment is to extract pigments from baby spinach leaves and purify products using column chromatography. Introduction: (3 marks)* Brief description of the concept/reaction studied – extraction, column chromatography – and why it is important Column chromatography is used to separate pigments based on their