Shear strength

of the in-service behaviour of buildings showed that this lack of strength did not necessarily result in building failure or even severe damage when they were subjected to severe earthquake attack. Provided the strength could be maintained without excessive degradation as inelastic deformations developed, buildings generally survived and could often be economically repaired. Conversely, buildings which experienced significant strength loss frequently became unstable and often collapsed. With this knowledge

Neutravidin Conjugation and Antibody Attachment After thiol functionalization, surfaces were treated with 50 µg/mL maleimide-activated neutravidin (Thermo) in phosphate buffer saline (PBS) for 1 hour at 37 C. The maleimide-activated neutravidin covalently attached to the thiol-functionalized surface through the maleimide-thiol coupling at neutral pH. Unreacted neutravidin was removed with three PBS washes and the substrates were stored in PBS at 4 C for up to one week before use. Biotinylated

within the reversibly sealed SEAM platform. SEAM allowed easy integration of nanotextured PDMS and enabled controlled application of fluid shear stress. EGFR is upregulated in several cancer types and is a common target for cancer cell capture. A human non-small lung cancer cell line, A549, was used to investigate possible enhancements in cell attachment strength. These cells were chosen because they have relatively low

As a woman on the brink of becoming a grandmother for the first time and working on my Paramedic license, I do not want to become a statistic and suffer from a back injury that so many first responders suffer from these days. There are so many variables to staying healthy such as eating right, staying fit, and learning proper mechanics for lifting heavy loads. When taking these variables into account, how can someone in my profession improve the quality of our work environment to insure a better

alter the shape of a loaded member and explain the possible effect of excessive stress on a structural member. Annotated sketches are essential. Scenario: A large derelict building is to be converted into industrial storage. The building contains an external hoist that was used to raise loads to the top floor of the structure. The building appears structurally secure but the local planning office requires evidence of some structural calculations before awarding planning permission. To the right

Thin walled structures are an important part of engineering construction with territories of use becoming diverse continuously ranging from girder bridges, oil vessels to industrial warehouses , framed structures. Thin walled sections have various stresses and failure modes which can be difficult to predict. Thus structural engineers need help of computers for analysis of these structures. This has been done by using software called THIN-WALL which estimates the cross-sectional

This lab demonstrated qualities of standing waves with great help from a mechanical vibrator, a sine wave generator, and a piece of string. Through this lab we were able to understand how to calculate the speed of a wave on a string. A standing wave is defined as a wave that travels forward, then is reflected creating nodes (regions with minimal to zero energy). If the frequency of a standing wave is increased then more loops will be created, and because of that the wavelengths will be decreased

predict the elastic deformation of a body. Simple stress can be classified as normal stress, shear stress, and bearing stress. Normal stress develops when a force is applied perpendicular to the cross-sectional area of the material. If the force is going to pull the material, the stress is said to be tensile stress and compressive stress develops when the material is being compressed by two opposing forces. Shear stress is developed if the

The requirement of a joint, which is the junction of beams and columns, is to enable the adjacent members to develop and sustain their ultimate capacity. The joints should have enough strength and stiffness to oppose the inner forces induced by the framing members. 1.2. CLASSIFICATION OF JOINTS A. Indian Standard Classification In a moment resisting frame, three types of joints can be recognized , interior joint, exterior joint and

CU1521-Support children and young people’s health and safety Task 3 Assessment criteria 3.3- give examples from own practice of supporting children or young people to access and manage risks. Whilst I was at placement I have taken part in a lot of activities. Some of these have needed me to tell the children how to work safely. One of the activities that I had to do this was when they were using the scissors. I assisted in an activity where they had to make poppies. This was done by making