Silica gel

     Change, like time, is always happening. There is no way to stop it, not even for a second. Whither or not you realize it, you are always changing in every possible way. However, we commonly simplify change to only the large differences in our normal routines each day or week, whither they are expected or unexpected. These large problems can sometimes become problems for people, which is not surprising. They should be problems, whither they are good problems to have,

SDS page Name: Shelby Clark Lab Partners: Kaliah Goodman, Howsikan Kugathasan, and Suntesia Bowen Date of Laboratory: September 21, 2016 Goal of Laboratory: The goal of this laboratory was to successfully make a gel and to run an SDS-PAGE and determine molecular weight (MW) of an unknown protein sample by comparing it to Log10 of the migration of molecular weight of the standards. Background: The purpose of SDS-PAGE is to separate proteins according to their size, and no other physical feature

4. Determination of Galacto-oligosaccharides The techniques used for the determination of GOSs should be simple, rapid and precise. So, range of methodologies have been used to determine the GOSs including, gas-liquid chromatography, thin-layer chromatography, high-performance liquid chromatography and other related techniques such as: Electrospray Ionization-Mass Spectra, Nuclear Magnetic Resonance, UV-spectrophotometry, High pH anion exchange chromatography with pulsed amperometric detection (Table

experiment is to perform a procedure known as SDS-PAGE (sodium dodecyl sulfate - polyacrylamide gel electrophoresis) in order to determine protein profiles for seven fish species: Yellow Tuna (Thunnus albacares), Thresher Shark (Alopias vulpinus), Paiche (Arapaima gigas), Swordfish (Xiphias gladius), Catfish (Ameiurus catus), Coho Salmon (Oncorhynchus kisutch), and Rainbow Trout (Oncorhynchus mykiss). The gel electrophoresis separates the fish muscle proteins according to their molecular weights, which

Materials and methods Chemicals used: Fipronil (Insecto SC 5%) is a product of BASF Company and manufactured by Sinochem Group- Ningbo Technical Co. Ltd, China. Zinc was obtained (in the form of zinc sulphate heptahydrate) from October Pharma, Egypt. The kits used for the following biochemical assays were purchased from Biodiagnostic Company, Dokki, Giza, Egypt: superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), glutathione-s-transferase

When examining the purification data in Table 4, we can analyze the different methods of purification of LDH and determine how effective each methods is in order to ultimately purify LDH. Unfortunately, there are two different definitions of purification: one is have a high end yield of the protein, while the other is having a high end concentration without any contaminating proteins. Furthermore, in order to achieve one type of purification, the other one has to be given up. The very first purification

The cation exchange gel samples are labelled IEX 19-22 as these were the tubes with the highest activity as shown in the above table. The other IEX fractions had significantly lower specific activities so were less likely to contain the protein of interest and more likely to contain unwanted proteins than the higher specific activity fractions. Only the higher specific activity fractions were chosen for this reason. All the fractions that were analysed by gel electrophoresis have the same banding

Gel electrophoresis is a gel technique that separates DNA and proteins based on their mass, by means of an applied electrical field that passes through an agarose or polyacrylamide gel. The concentration of agarose in the gel is commonly 0.8% to 1.0%, since agarose is expensive. The gel is embedded on a buffer, pH of 8.3, which keeps the pH of the solution at equilibrium. Assuming that at typical pH, DNA is negatively charged, denatured protein samples are placed in the wells located on the negative

through the polyacrylamide gel. The next step is to put the gel into the electrophoresis module and to run it. It is run until the proteins have almost reached the bottom of the gel. A blue tracking dye is added to the Laemmli sample buffer in order to track the distance in which the proteins travel through the gel. If it is run for too long, the proteins will run off the bottom of the gel and it will mess up your results. Once the protein reach the bottom of the gel, the gel is stained in order to

Blood Exo DNA ProTeck™ Vacutainer blood collection tube for stabilizing extracellular vesicle DNA in a whole blood sample Research Use Only. Store at room temperature (18 to 25⁰C) Catalog # 0019273 9 × 10 mL tubes 0019273 100 × 10 mL tubes Intended Use Blood Exo DNA ProTeck™ is a 10 mL vacutainer blood collection tube for stabilization of extracellular vesicle DNA in a whole blood sample at room temperature for at least 30