Single domain antibody

Abstract Emeria acervulina ovarian tumor (Ea-OTU) belongs to OTU domain class of cysteine protease deubiquitinating enzymes. We show here that Ea-OTU is highly linkage-specific, cleaving Lys48(K48)-、Lys63(K63)-、Lys6(K6)-linked diubiquitin but not K29-、K33、K11-linked diubiquitin. Recombinant Ea-OTU but not a catalytic-site mutant Ea-OTU (C229A、H359A) exhibited activity against diubiquitin. The activity was selective for diubiquitin chains with preference for specific lysine linkages (K6 >K48>K63)

Acute lymphoblastic leukemia(ALL), also known as lymphoblastic leukemia/lymphoma in the WHO classification, is a malignant expansion of immature lymphoid cells that results from multi-step genetic changes in a single lymphoid progenitor cell. Its incidence peaks between the ages of 2 and 4 years(1(. Acute lymphoblastic leukemia (ALL) is the most common malignancy in children (2). ALL causes 77% of children leukemia, and acute myeloblastic leukemia (AML) counts for 11% (3). ALL is categorized

In tandem with this I could also engineer a longer flexible linker between the two domains of the CLAMP-dCas9 fusion to supply the CLAMP domain with more space to move around and interact with other factors. This would allow for additional flexibility in spacing for targeted guide RNA sequences. After increasing the density of CLAMP in varying amount I will again

Use of Genetic analysis to study the nature of the Smyd1b gene in Cardiac and Skeletal Muscular Systems of Zebra Fish Prajwal Keranahalli Poolesville High School Institution: Institute of Marine and Environmental Technologies/ University of Maryland Baltimore Campus Mentor: Dr. Shaojun Du Research Project Teachers: Mr. Mark Curran, Dr. Patricia Miller Summer 2014 Abstract: One of every 5600 to 7700 males below 30 suffer from genetic muscular dystrophy. Muscular dystrophy is a group of genetic diseases

Herpes simplex virus (HSV) is one of the most prevalent human viral infections worldwide. It belongs to the family Herpesviridae and is composed of linear double-stranded DNA surrounded by icosahedral capsid (Uyangaa et al., 2014). There are two types of herpes virus, namely HSV type 1 and type 2 (HSV-1 and HSV-2), which are the common cause of oral (cold sores) and genital herpes. The HSV infection is highly contagious and can be transferred from person to person through direct close contact. In

clonal evolution model states that cancer cells develop from normal cells that accumulate a series of mutations which give them growth advantage under selective pressure. Genotyping of colorectal cancer has limitations; tissue samples only provide a single snapshot in time which prevents monitoring of tumour progression and there is spatial selection bias due to tissue heterogeneity. Tissue heterogeneity, which can be characterised as distinct morphological and phenotypic profiles between tumour cells

life quality due to the inability to work and exercise over its attacking4. It is life-threatening for severe cases and 419 Australian died of asthma in 20146. The development of Asthma is closely related to immunoglobulin E (IgE), a subtype of antibody molecule that normally response against antigen stimulation and induces various immune responses5. When exposed to specific antigens, IgE is released from B lymphocytes and binds to its receptors on immune effector cells (e.g. mast cell, basophil

• Demographics: 8 year-old male ♂ (Dorian Gray) • Chief Complaint: painful ulcers in oral mucosa and swelling/redness in his right hallux (“big toe”), weight loss, abdominal pain, initially was constipated but now passing stools regularly with no observable blood, elevated temperature, poor appetite, fatigue, skin lesions on right shin, painful sensations in jaw • History of Current Illness: No prior history of current illness before 8-years of age • Medical History: • Current medications: none

costimulatory molecules or cytokines can enable engineered T-cells to eliminate tumor cells. CARs are designed in a modular fashion and generated by fusing the antigen-binding region of a monoclonal antibody (mAb); extracellular target-binding domain to a transmembrane domain and intracellular-signaling domains. They have shown clinical benefit in patients treated with CD19-directed autologous T-cells. Recent successes suggest that the modification of T-cells with CARs could be a powerful approach for

enzyme. Viral variants may develop at the time of this process because reverse transcriptase is error prone. During the midpoint of infection, both the viral protein integrase and the host DNA repair enzymes will inject the viral genome into the active domain of the host’s chromosomal DNA. Lens epithelium-derived growth factor (LEDGF/p75) is an integrase binding host factor that assists the progress of integration, which converts the cell into a virus producer. In the late stages, production of viral particles