Solution

Afterward, PTEN purified using affinity chromatography. Since PTEN fused with His6 tag, Ni-NTA affinity chromatography would work to purify PTEN. Ni-NTA is a type of immobilized metal affinity chromatography that contain coupled Ni2+ nickel chelate forming ligand (imiondiacetic acid) that immobilized to agarose. The science behind using Ni-NTA affinity chromatography is that histidine bind specifically and reversibly to the nickel chalet thus washing step will remove other contaminate and then His-PTEN

The Effect of Solvent on the Ability of Solute to Dissolve Problem: How does the solvent effect the ability of a solute to dissolve? Hypothesis: If the solvent is denser, than the time it takes the sugar to dissolve will decrease. Materials: Sugar Rubbing Alcohol Vegetable Oil Water Scoopula Beaker(200mL) Graduated Cylinder(50mL) Stirring Rod Balance Stop Watch Medicine Cup Procedures: 1. Measure 25mL of water into a graduated cylinder. 2. Pour the water into a beaker. 3. Measure 2g of sugar

Experiment 5: Liquid CO2 Extraction of D-Limonene from Orange Rind Lela Gregory Partnered with Madison Pretto TA: Sylvia Singh Introduction Liquid carbon dioxide extraction, also called CO2 liquid extraction for short, is a method of separation and extraction frequently used in the laboratory setting. The processes uses phase changes that occur due to pressure and temperature changes to its advantage to allow for the extraction to occur1. The phase change allows for components of

We had to do an egg drop so when it is dropped from 20 ft it wouldn’t crack.. But I really didn’t have any background knowledge on egg drops because I never did one. But I did know that by putting a plate on the bottom it would make it float down and not fall. So when it was dropped from 20ft it wouldn’t crack. The highlights of the project was the design it was a really good design so when it was dropped it didn’t crack the egg. The downers of the egg drop was if we had more and better materials

1. Allow the seeds to germinate for three days prior to the experiment. Prior to the first day, soak them in water overnight. On subsequent days, roll them in a moist paper towel and place the towel in a paper bag. Place the bag in a warm dark place. Check each day to be sure the towels remain very moist. If time is short, the peas can be used after they have soaked overnight. For best results, allow them to germinate for the full three days. 2. The recommended numbers of peas for use in this experiment

Bubble Research What is a bubble and how does it from? Bubbles are a pocket of soap and water that floats in the air. Bubbles form when soap and water mix together. air is blown into it. The soap trap the air and creates a bubble. You can see bubbles in liquid like water. Bubble are in everything you drink. Bubbles don’t have to form with soap. Bubbles in drinks can’t float freely in the air. Only bubbles with soap can flow freely in the air. What is the science behind how bubbles form? When soap

Salts are used in many different environments and for many different reasons. One common purpose that most people know of is the use of salt on the road to help the roads not freeze over in the winter. People use salt for things like this because salt has the ability to lower the freezing point of the water. This happens because the salt dissolves in the water and the moving water molecules begin to “melt” the ice. Since there are molecules of solute in the way, the formation of the ice crystals

When one is involved in outdoor activity, the weather and environment is not to be taken lightly. Cold injuries will happen when the weather conditions are cold, windy, or wet. Cold injuries are classified into 3 categories: decreased core temperature (hypothermia), freezing injuries of the extremities, and nonfreezing injuries of the extremities.1 Hypothermia (core temperature below 95°F) is classified as mild, moderate, or severe, depending upon how low the core temperature is measured.1 Hypothermia

Salinity Experiment Introduction: As you now know, the ocean contains many dissolved salts. How do you think this may affect the objects within the ocean? Have you seen some objects float, while others sink to the bottom? This is directly related to density, which is a measurement of the mass per volume of an object. Density determines whether an object will float or sink. But how are salinity and density related? In this activity, I will investigate the effects of salinity on density by observing

Conclusion: For our hypothesis we thought that the seltzer would dissolve the alka seltzer the fastest, because the seltzer was already carbonated. After doing the experiment we found out that the seltzer was the 2nd fastest at dissolving the tablet, and the water dissolved the alka seltzer 6 seconds faster than the seltzer. This being said our hypothesis turned out to be wrong because the water (fastest to dissolve the alka seltzer) has fewer chemicals, so it dissolved the tablet faster