Southern blot

Aphids possess bacteriocytes that are specific to its obligate mutualist, Buchnera aphidicola. It seems that this genus has lost genes that are essential to its life. Through Southern Blot analysis, Nikoh and Nakabachi have shown that these lost genes are actually encoded in the aphid genome. Through detailed structural and phylogenetic analyses, the full-length sequences of these transcripts were determined. This demonstrated that these transcripts are indeed significantly similar to multiple bacterial

research with Simian virus 40 where he developed the Northern Blot technique, also known as the RNA blotting technique, at a laboratory in George Stark in the Biochemistry Department of Stanford University. It was named after the Southern Blot technique, which blots for DNA, and the Western Blot technique, which blots for proteins. It is not commonly used for clinical or diagnostic purpose but it is mainly used in research. The Northern Blot technique allows scientists to determine the molecular weight

Guy11 was used as wild type of M. oryzae for transformation in this study. Mycelia harvested from liquid CM was used for DNA and RNA extractions. For vegetative growth, small squares of mycelia were cut from the edge of 7-day-old strains and placed onto the media (CM, MM, OM and SDC) and incubated in the dark at 28°C for another 7 days. For conidia collection, mycelial blocks were inoculated on SDC (100 g of straw, 40 g of corn powder, 15 g of a gar in 1 L of distilled water) agar media [50] maintain

Goffman (1963) defined stigma as any condition, attribute, trait, or behavior that is deeply discrediting and reduces the bearer from a whole and usual person to a tainted and discounted one. . Stigma consist of an attributes that marks people as different leading to devaluation. Stigma is socially constructed (Major & O’Brien, 2005) thus is dependent both on relationship and context. In fact, Goffman (1963) emphasized using the “language of relationships” (p. 3) when considering stigma. A subsequent

Nematodes were cultured and handled at 20 °C using standard methods (Brenner, 1974). C. elegans strains used in this study are as follows: N2 Bristol and MR156: rrIs01[Pelt-2::GFP; unc-119(+)] X, which were used as wild type. CB3514: lin-23(e1883)/dpy-10(e128) II, YHS47: lin-23(e1883)/dpy-10(e128) II; rrIs01[Pelt-2::GFP + unc-119(+)] X, DH2: zyg-11(b2) II. DS97: mat-2(ax76) II, EU593: mel-26(or184) I, EU640: cul-2(or209) III, UP63: mat-3(ku233) III, NJ582: cul-1(e1756)/unc-69(e587) III, VC141: zif-1(gk117)

Proteomics is the study of protein and how they interact and function within organisms. This results in catalyzing and cataloguing proteins; while comparing how each variation of protein expression in different conditions. This is done in a large scale manner; a good example of this is the Human Genome Project that took place from 1990 to 2003. With the field of Proteomics it has given the ability to understand genes and to comprehend why there’s so much diversity of protein, including their importance

Immunoblotting is an effective approach that utilizes antibody-tags to analyze, isolate, and quantify proteins. A Western Blot, a type of immunoblotting method, was used in this experiment to evaluate the protein quantities contained within the different subcellular fractions. Although, the Western blotting method can be implemented for the study of more complex cellular processes. Complex cellular processes such as metabolism can be greatly evaluated with immunoblotting methods. For example, some

Imagine a world where an innocent child is given HIV due to mother to child transmission. Imagine a world where a father cannot play football on an autumn’s afternoon with his son because he constantly feels a lack of energy. Imagine a world where for months straight one has a reoccurring yeast infection. And lastly, imagine a world where one cannot remember one’s very own mother’s name due to short term memory loss. Unfortunately, there is no need to imagine for all of the above are actual possibilities

Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease

1) I would like to become an HIV Peer Test Counselor because I want to empower students with the knowledge and agency to live healthy lives as they balance their sexual, emotional and physical health. Taking an HIV test is an intimidating experience for many people, so I want to provide my peers a warm, anonymous, and safe space where they can share their experiences while I help them make informed decisions that reflect their cherished values and beliefs. This role will help me increase my emotional