The staining procedures used to differentiate the types of bacteria are known as differential technique of staining. The simple methods of staining impart same color to all types of bacteria and other biological materials. The principle behind this differentiation is due to the variation in physical and chemical properties of the cell. Consequently, reaction occurs differently to reagents. In handling of the sputum when testing for the presence of bacteria pathogen one has to ensure that there is
pH 6 for a total 20 min. Non-specific staining had been blocked by superblock (UV block) for 10 minute. Sections were incubated with the primary antibodies for 60 min. The antibodies used were galectin-1 (Genemed, clone NBP2,CA, USA, diluted at 1:400) and galectin-3 (Genemed, clone 9C4, CA, USA, diluted at 1:100). Secondary staining kits were used according to the manufacturer's instructions (Thermo scientific corporation Fremont, CA, USA). Counter staining was done with
Lab Title: Differential Staining- Visalization of Bacterials Cell Strutures Lab The Aim of the lab: the purpose of the lab was for students to be able to differential between endospore and vegetative cells by looking at their cell sturtures. Materials: • Glass microcope slides • Bursen burner • Inoculating loop • Sharpie pen • Sterile Water • Beaker with boiling water • Malachite green ( primary stain). • Safranin (counterstain) • Bibulous paper Method: • Prepare smear as directed by professor
Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
Gram Staining Formal Lab Report Miranda King Intro Microbiology Dr. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. This technique uses crystal violet stain, Gram’s Iodine, Ethyl Alcohol, and Safranin. These dyes are used in order to distinguish between the different types of cells. For example
bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive. Introduction The purpose of this lab was to view the different characteristics of bacteria by applying various staining techniques. It is important to know the
Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus, Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded, clusters
examine their reaction to certain dyes and determine whether the culture is negative or positive, it’s morphology, and arrangement of the cells. By using the Gram staining method, microorganisms can be narrowed down for the identification process as well as the leading to diagnosis Procedure: For this experiment, we were given three gram staining slides as well as a petri dish with five different types of incubated microorganisms that were divided. The five different organisms on the petri dish being
MICROBIAL SURVEY, SMEAR PREPARATION, AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green, liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae
Theories of Staining Techniques Staining bacteria with different dyes via staining techniques, allows in distinguishing the microorganism from its backgrounds. Also, helps in studying different internal structures such as vacuoles, cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining, endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also, these staining procedures help in determining properties