Staining

we have learned during this course. It was achieved, by preparing a gram staining procedure, to help us identify the morphology of the bacteria, and to prepare a various of different test, such as the starch test, oxidase, urease, citrate, MSA, Nitrate Reduction and MP/VP to help us identify if the bacteria is able to produce the specific catabolism that it being testes for. Procedure: Gram Staining             Gram Staining is a procedure used to distinguish between gram-positive and gram-negative

Tiffany O’Connor 9/2017 Gram-positive and Gram-negative stain Purpose: Staining is done to differentiate between two varieties of bacteria, gram-positive and gram negative. Theory and Background: Gram-positive and Gram-negative represents whether a cell wall retains the primary dye color in the staining process after decolorization. The difference in the structure of the cell wall determine if this happens. In Gram-positive cells the walls are much thicker than Gram-negative. Gram-positive cell

Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian's gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria

There are numerous reasons why it is essential that gram stain and microscopic examinations are the first step in identifying pathogenic bacteria. Gram stains are an important procedure because it can determine the stain of the cell wall of bacteria sample. It also allows laboratory technicians to classify the bacteria in to either gram positive or negative as well as allowing them to analyse and study the morphology and arrangement of bacteria. Whilst determining the bacteria type and type of gram

bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive. Introduction The purpose of this lab was to view the different characteristics of bacteria by applying various staining techniques. It is important to know the

Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus, Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded, clusters

examine their reaction to certain dyes and determine whether the culture is negative or positive, it’s morphology, and arrangement of the cells. By using the Gram staining method, microorganisms can be narrowed down for the identification process as well as the leading to diagnosis Procedure: For this experiment, we were given three gram staining slides as well as a petri dish with five different types of incubated microorganisms that were divided. The five different organisms on the petri dish being

celled prokaryote microorganisms, rapid mutations causes bacteria can be found just about anywhere.Gram staining helps to classify two groups of bacteria, Gram-positive and Gram-negative. Gram-positive bacteria's cell wall has a stronger attraction for crystal violet, because of more peptidoglycan. Gram-negative is a group of bacteria that doesn’t retain the crystal violet stain used in the Gram staining method, making positive identification possible. These are the many characteristics used to describe

Theories of Staining Techniques Staining bacteria with different dyes via staining techniques, allows in distinguishing the microorganism from its backgrounds. Also, helps in studying different internal structures such as vacuoles, cell walls and spores in details (Seeley and others 1991). Some staining techniques such as Gram staining, endospore stain and capsule stain are some of the theories of stains used in bacteriology today. Also, these staining procedures help in determining properties

MICROBIAL SURVEY, SMEAR PREPARATION, AND SIMPLE STAIN Instructional Objectives 1. Define Roccal = green, liquid disinfectant. Pathogen = an agent which causes disease. Wet Mount Slide = a microscope slide of a liquid specimen covered with a cover glass. Yeast = a single celled fungi. Budding = a true characteristic method of asexual reproduction among yeasts where budding of a new cell from a parent cell can be observed. Mold = multicellular masses of filamentous fungal growth. Hyphae