Taq polymerase

The main goal of our group project was to use a series of different laboratory techniques to build a phylogenetic tree, or a graphical representation of the evolutionary lineage and relations of a set of organisms. Essentially, we aimed to isolate the 5.8S rRNA (a small RNA molecule found in the ribosome) in a collected specimen. After collecting our samples on a field trip, we analyzed their physical characteristics. I chose to collect a sample of a purple flower. A few defining characteristics

The chromosomal DNA was digested with different dilutions of Bam H1 (10units/µl). The reaction mixture consisted of 10 g of chromosomal DNA and 10 l of the restriction enzyme buffer in a final volume of 100 l. In the first PCR tube 20 l of the reaction mixture was dispensed while 10 l was dispensed in 2 to 7 PCR tubes. The tubes were placed on ice for 2 mins and 10 units of the Bam H1 were added to the first tube. The contents were mixed and 5 l from the first tube was transferred to the second

Real-time PCR detection of HIV viral load Introduction: HIV, the Human Immunodeficiency Virus is a lentivirus which is responsible for HIV infection and eventually causes AIDS. It’s assumed that the virus has been in existence since 1930 but it’s still unclear that how the virus came into existence. There are two kinds of HIV virus, HIV-1 which causes more severe disease and the source of transmission is a chimpanzee species Pan troglodytes whereas HIV-2 is transmitted by Cercocebus atys, a monkey

Subject identification and enrollment Subjects recruited for this study consisted of individuals aged 60 to 85, with 10 or more years of education or more. Those diagnosed with Alzheimer’s disease (AD) and mild cognitive impairment (MCI) were recruited to the study at the Memory Clinic of the Jewish General Hospital (JGH) in Montréal, and normal community elderly volunteers were recruited through advertising. Subjects received standardized clinical, mental status, neuropsychological, and

abnormal alterations in regulation that may result in a disease state quantification of transcription levels of genes plays a central role. Molecular medicine and clinical diagnostics progression crucially depends on the innovation of the real-time polymerase chain reaction (PCR) technique. For example we can name the quantization of relative gene expression as detection of minimal residual disease and cancer diagnostics (Zaros et al.,

Results Orchard inoculation studies In the orchard studies of 2014 season, significant variations (P<0.05) on disease severity (Table 4). Disease severity increased with increasing conidial concentration. The conidial concentration of 5000/ml was minimum for 1% disease severity in mature detached fruits in lab. The interaction between inoculation date and inoculum concentrations indicated the dependency of disease development on growth stages of fruits besides conidial concentrations (Fig. 5)

to Sanger sequencing (Retrogen, Inc. San Diego, and CA). The sensitivity of RT-PCR technique for detection of PMTV from soil extracted RNA was determined by using total RNA extracted from series of spiked soils. Real time reverse transcription polymerase chain reaction (qRT-PCR) Attempt was also made to detect PMTV from the soil extracted RNA using qRT-PCR. The primers and probes were designed to target coat protein read through protein gene with the help of sequences obtained from the NCBI GenBank

Summary Bluetongue virus (BTV) is an arthropod borne virus causing ruminant haemorrhagic disease (MacLachlan, Drew, Darpel & Worwa, 2009). Typical presentation includes fever, mucosal erosion, ulcerations, and coronitis (MacLachlan, Drew, Darpel & Worwa, 2009). BTV has 24 serotypes, many topotypes, and can rearrange its double-stranded RNA genome to further increase variability. In 2006 an epidemic of BTV serotype 8 (BTV-8) caused a surprising amount of foetal infection. Consequently, this study

to Sanger sequencing (Retrogen, Inc. San Diego, and CA). The sensitivity of RT-PCR technique for detection of PMTV from soil extracted RNA was determined by using total RNA extracted from series of spiked soils. Real time reverse transcription polymerase chain reaction (qRT-PCR) Attempt was also made to detect PMTV from the soil extracted RNA using qRT-PCR. The primers and probes were designed targeting coat protein read through protein gene with the help of sequences obtained from

1. Plasmid DNA -Plasmid is a small, circular, double-stranded DNA molecule that naturally exist in bacterial cells. The range of length are from hundreds base pairs to thousands base pairs. This is a great tool for clone, transfer, and manipulate genes. Figure 1: Plasmid DNA 2. Sticky Ends, Blunt ends -Two different results after restriction enzymes cut DNA. The Blunt ends has no overhang on one strand or the other. The sticky end is that each strand extends beyond the complementary region of