Taq polymerase

The bacterium Thermus aquaticus is a fascinating organism that is utilized for a revolutionary process called a polymerase chain reaction (PCR.) The bacterium was discovered in the hot springs of Yellowstone, and is generally found in very hot climates, typically not livable by other bacterium. The bacteria belongs to the Archaebacteria kingdom, is a chemoautotroph, and is typically cylindrically shaped, but can be rod shaped or spherically shaped. PCR is a tool used by those looking to amplify

Introduction Finding DNA in a tissue is very essential for any experiment anyone can do. Looking for a certain species can help identify dead bodies, animal type, and also the source of meat used in foods you eat According to United States Government Accountability Office 48 million people become sick from things you put and cook in food (United States Government Accountability Office 2013, pg. 3) For example, Taco Bell has used horsemeat in their foods for years and this experiment proved it

The second technique used in gene therapy is ligation, which is used to ‘glue’ the removed functioning ADA gene into the viral DNA so that recombinant circular DNA (cDNA) can be created. Viral DNA is obtained from a virus, such as an adenovirus (often used for gene therapy treatments) which has been ‘purified’ so that it does not cause harm (infect host with the virus) to the human body when introduced. The ADA gene is then spliced into the open vector (viral cDNA), using the enzyme ligase which

Introduction Genes are unique segments of DNA and in this laboratory experiment, the Actin gene of Drosophila melanogaster will be extracted and amplified with various laboratory processes including PCR, ligation, and transformation. Also, the gene that was extracted will be confirmed and sequenced with the process of cycle sequencing and with the help of NCBI database. The DNA that is first extracted will be referred to as “genomic DNA” because it was extracted directly from the fruit fly, but

is the best between multiple things (if it even is) and why. That is why we do it, ultimately. We feel that we must answer the question “Why?” In this biographical paper, I will be analyzing two very different processes: DNA Replication and the Polymerase Chain Reaction (PCR). It is that each of these individual processes carries much importance. DNA replication is important in the life of a cell, more so the division, because when a cell divides both of the daughter cells need identical DNA to function

1. In your own words, describe what the field of bioinformatics is; write one paragraph (about 300 words) about this; be very descriptive. The field of bioinformatics is the process of technology by the use of computers (BioPlanet, 2015). This field manages biological information (BioPlanet, 2015). We all use computers on a regular basis, but computers are capable of much more they can store biological information, gather this information, analyze and integrate genetic information as well (BioPlanet

DNA is like the blueprint for the creation and proper functioning of every living organism. Organisms can sometimes be divided into prokaryotes and eukaryotes. Examples of prokaryotes and eukaryotes include bacteria and humans, respectively. These organisms must possess a method of replicating DNA, so a copy is provided for each cell that divides. Each cell’s responsibility is coordinated by the piece of DNA and thus, makes it a very valuable part of the cell and organism. So what are the methods

Yellowstone National Park is a truly unique place. Few places in the world contain such amazing biodiversity, and such a wide variety of species in such a condensed area. While many scientists have been interested in studying the various plants and animals that inhabit Yellowstone, a group of microorganisms that has been of special interest in the past few years are Yellowstone’s extremophiles—the microorganisms that inhabit Yellowstone’s thermal springs. Extremophiles have opened the eyes of science

Ch 20 PDQs 1-14 How do restriction enzymes work? After recognizing the restriction sites, the restriction enzymes cut off the DNA strands at these points, leaving sticky ends on the original DNA fragment. The restriction enzymes can also travel to other cells to cut off DNA sequences, the sequences that can match the bases of the original sequence. The sticky end will find other sticky end to form a new recombined DNA sequence, obeying the law of base-pairing. Explain the significance of sticky

It is used to amplify a sample of DNA, creating thousands or even millions of copies of a certain sequence of DNA. "The story of modern PCR begins in 1976 with the isolation of Taq polymerase from the thermophilic bacterium Thermus aquaticus" (https://www.thermofisher.com/uk/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/history-pcr.html).