Taq polymerase

Alu elements are non-coding DNA that are named after a recognition site for Alu I. Alu insertions results from transposable DNA, which insert copies of themselves into new chromosomal locations. These insertions are important in reconstructing the human prehistory by comparing allele and genotype frequencies between modern populations. Most Alu insertions are fixed, but recent studies have found dimorphic insertions within the last million years. Fixed means that both chromosomes have the insertion

ASSIGNMENT 1: PROPERTIES OF DNA/RNA Introduction: The polymerase chain reaction is an innovative technology, which amplifies a single piece of DNA across several orders of magnitude. The end result is the creation of thousands to millions of copies of a particular DNA sequence. PCR is closely patterned after the natural principle of DNA replication. It is a three-step process, referred to as a cycle, that is repeated a specified number of times. One PCR cycle consists of the following steps:

ignored. Biological Background: It is scientifically proven that DNA (deoxyribonucleic acid) contains genetic material and highly specific to individual. DNA fingerprint can be tested by restriction fragment length polymorphism (RFLP) methods and Polymerase chain reaction (PCR) methods. Although RFLP is considered more accurate, due to the cost and requirement of longer period to complete, it is not commonly used. While only 1% of genetic materials are unique to individual, the short tandem repeats

carried out using two possible methods: polymerase chain reaction, or PCR, and restriction fragment length polymorphism, or RFLP. To begin either method, DNA must be isolated from blood, semen, vaginal fluids, hair roots, skin, skeletal remains, or objects. In the process of PCR, small samples of DNA could be used because PCR utilizes DNA’s unique ability to make many copies of itself. Like DNA replication within an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using

synthesis were PCR amplified using pFx enzyme respectively for both premature AI and mature AI. The products of the PCR have blunt ends due to the amplification done by pFx. Mature AI was not able to be amplified using pFx so it was done using Taq Polymerase. PCR clean up of the products were done and was quantified using Nanodrop. Restriction digestion of the Mature AI, Premature AI and pET28-a was done at the Xho and Not1 restriction sites. Purification of the digested products were done using Sodium

DNA Technology Task 3 – Poster DNA fingerprinting, also known as DNA profiling, is a technique used in forensic science that identifies individuals based on various characteristics of their DNA. Although the DNA sequences between humans are 99.9% identical, DNA fingerprinting is able to distinguish between individuals due to the presence of specific sequences within the non-coding region of the genome known as satellite DNA. This satellite DNA consists of long stretches of DNA made up of repeating

Short tandem repeats, also known as STR, are microsatellites found on a DNA strand that aid in forensic analysis. In forensics, the focus falls on Y-STR, as in, Y chromosome STR and Autosomal STR. Y-STR are only found on the male Y-chromosome and autosomal can be found on the chromosomes of men and women. Despite STRs being found in both sexes, they are vastly variable among individuals, due to inheritance from each parent, varying in size. This high power of discrimination is vital for forensic

Transgenesis Transgenesis is the process in which an exogenous gene (transgene) is added to an organism’s genome, and it results in expression of the gene, which can then be passed down to its offspring. [1] I have chosen to research how enviropig has been manipulated using transgenic techniques. The transgene inserted into the enviropig’s genome is Escherichia coli K12’s phytase gene, this gene gives the enviropig the ability to produce phytase in their salivary glands, and it is infused in their

At a crime scene, insects can provide important pieces of information to criminal investigators, including estimating the post-mortem interval (PMI) and determining where the crime took place. This is only possible, however, if the local insect fauna is known, thereby facilitating the need for local insect population surveys at both the morphological and gene levels. Although flies (order Diptera) are one of the first and most prevalent necrophagic insect species found associated with a corpse

Introduction  In this lab my class mates and I studied the process used in DNA fingerprinting known as gel electrophoresis. We used substitute chemicals instead of DNA to demonstrate the process of this. electrophoresis is the process in which DNA can be analyzed using tandem repeats. These are the 4 base pairs that repeat in the same order for a certain number of times. The number of times these base pairs repeat can be seen when the DNA is run through the electrolysis chamber. The DNA is