TBE buffer

Bacterial tannase enzyme were purified from the 24 h old solid state culture filtrate of E. cloacae by two-step purification protocol consisting of ammonium sulfate precipitation (60%) followed by chromatography techniques, Ion exchange chromatography (DEAE) and Gel filtration chromatography (Sephadex G-100). In the final step, Ion exchange chromatography enzyme was purified 1.96 fold with 20.25% recovery and specific activity of 5.51U/mg, and then gel filtration chromatography enzyme was purified 1.45 fold

The buffer is a solution that minimises the changes in pH when small amounts of acid or base is added.There are two types of buffer solutions.They can either be acidic or alkaline.An acidic buffer is made by mixing equal amounts of a weak acid and its conjugate base.Similarly, an alkaline buffer has equal amounts of weak base and its conjugate acid. Alka seltzer acts as an antacid. It uses sodium hydrogen carbonate to neutralise excess hydrochloric acid in the stomach and thus relieves heartburn

Introduction: A buffer solution is a solution (consisting of a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid), which minimises changes in pH when small quantities of acids or bases are added to it. In a way, it is used to consistently moderate the pH of a solution, and is measured using the Acid Dissociation Constant. General Formula: HA⇋H^++A^- NaA⇋N^++A^- Where HA is a weak acid, N+ is a salt, and A- is a weak base. Example of a basic buffer: 1.0 M of NH3 solution

catechol oxidase, extracted from masticated potato (Solanum tuberosum) lowers activation energy, as it is a catalyst. This enzyme can react with catechol to produce benzoquinone and water. Catechol oxidase is tested against a multitude of phosphate buffers, acidic, neutral and basic pH values, and chilled temperatures to hot temperatures. The purposes of these testes were to determine the optimal temperature and pHs at which catechol oxidase performs at. The method to measure results was the usage of

Hydrogen is a promising energy source due to its clean product and high-energy yield (122 kJ/g) (Chang and Lin, 2004). Water electrolysis, steam reforming of hydrocarbons and auto-thermal processes are well known technologies for H2 production, but it is cost-intensive due to high-energy consumption (Ming et al., 2008). In contrast, dark fermentation process is low cost technology for hydrogen production from waste materials (Tawfik et al., 2012, Tawfik et al., 2013 and Tawfik and El-Qelish, 2012)

CSci530 Computer Security Systems report: A brief review for Electronic Commerce Security Zhiheng Chu 4398125439 zchu@usc.edu I have read the Guide to Avoiding Plagiarism published by the student affairs office. I understand what is expected of me with respect to properly citing sources, and how to avoid representing the work of others as my own. I wrote the material in this paper, except for such material that is quoted or indented and properly cited to indicate the sources of the material. I

ITECH 3215 INFORMATION SECURITY MINOR ASSIGNMENT THREAT PROFILING VIVEK CHARY DADUVAI (30312832)  Contents Summary 3 Introduction 3 Profile of Threat 3 Profile Completion 4 Situational crime Prevention 4 Law 6 International scope 6 Conclusion 7 Reference 8   Summary Computer security is the security applied to the computers and their networks including the internet. Physical security and information security are the two types of computer securities which prevent theft of equipment

Additional roles of carnosine beyond Ca2+ regulation and pH buffer include its ability to act as a scavenger of free radicals and singlet oxygen, both of which are not only damaging, but also limit performance (Kohen, et al.,1988). Regarding BA supplementation and administration, there has been some opposing views

Effects of pH on Enzyme Concentration Abstract: In this lab report you will be testing the effects pH has on the rate on enzyme reactions, you will be collecting data and evaluating things that went will and ways it could be done better to help us further understand this lab. Research Question: How does pH affect the amount of reactivate when reacted with Hydrogen peroxide on an enzyme? Hypothesis: The higher the pH the higher the reaction with Hydrogen Peroxide on the enzyme will increase. Background

Most of the optimizations have focused on PH, solid liquid ratio, temperature and time, while no report on the effect of extractant concentration on pectin extraction. Therefore this research assesses the effect of extractant concentration and extraction temperature on pectin yield and characterization. Sweet potato pectin extraction conditions have been optimized by some researchers (Zhang and Mu 2011a; Zhang et al. 2013). The pectin yield and galacturonic acid content for the optimized condition