TBE buffer

Avi Borad The Effect of pH on Enzyme Activity Introduction: Metabolism is a necessary process in the function of living cells, which is driven by enzymes. Enzymes are catalysts that speed up chemical reactions by lowering the activation energy. Changes in the pH of an environment in which the enzymes act affect their performance capability. The optimum pH is the pH in which the enzymes catalyze the reaction at the fastest rate. The type of enzyme affects its optimum pH. Enzymes are typically denatured

used for each experiment. Catechol, potato juice, pH 7 phosphate buffer, and stock potato extract 1:1 will be used to conduct the following experiments: temperature effect on enzyme activity, the effect of pH on enzyme action, the effect of enzyme concentration, and the effect of substrate concentration on enzyme activity. For the temperature effect on enzyme activity, three test tube were filled with three ml of pH 7 phosphate buffer and each test tube was labels 1.5 degrees Celsius, 20 °C, and 60

contain. I am then going to give an explanation of water within the body, the location and the function of five electrolytes that are found within the body and the importance of maintaining this balance. I am finally going to include how the three buffer systems help to control the pH within the body. Distribution of

Overview: Citrate and its various salts have been researched extensively and found to be used in most commercial settings, such as a local supermarket, as food preservatives. Hence, the level of citrate salts of various metals in our daily food has to be allocated to acceptable levels; as well enforcement of these acceptable levels requires an analytical tool capable of quick, selective and sensitive detection and quantification of citric acid levels in our daily food. This analytical method utilizes

lemon juice, and 50 mL of household bleach all in separate 250 mL beakers are to be used. For part C, a hot plate or ice are to be used to make the 1.0 mL assigned temperature specific water. This experiment will also use the 1.0 mL of 0.1 Phosphate buffer. Procedure/Methods The procedures for experiment A, B, and C all start the same. The first step is to put on goggles and get the data collection device set properly. The labquest needs to be plugged into the colorimeter accurately so that a click

Objective The purpose of this experiment was to determine the pKa of the bromothymol blue (indicator) through absorption spectroscopy. Bromothymol blue being a monoprotic acid base indicator, displays different colors at different pH because of the differences in the ratio of the conjugated acid and base form. The fraction of conjugate acid and base was interpolated for the solutions through the acquired absorbance spectrum of the bromothymol blue at various pH. The rearranged form of Henderson Hasselbalch

RESULTS & DISCUSSION For this experiment, titrations on a weak acid, acetic acid, and a buffer were performed. Acetic acid was titrated with NaOH in order to observe the half-equivalence point as well as the equivalence point. Then, the buffer and the buffered acetic acid solution prepared faced additional titration with NaOH and HCl to evaluate the differing buffering effects following the addition of a strong acid and strong base. Finally, the buffer’s buffering capacity was calculated. If the

Practical 3 – pH, Buffers and Determination of pKA for a Weak Acid. Introduction This experiment was designed to test the pH changes of a weak acid, acetic acid, when it is titrated with a strong base, NaOH. We conducted this experiment so that we could obtain a pH titration curve and from this, we can correlate and signify the relationships between pH, pKA, and the ratios between the acetic acid and NaOH. This practical was key to developing our understanding of buffers and how they accept and

sample of acetic acid and the equivalence point is determined. Part two of the experiment consists of tested the dissociation constant. Acetic acid is mixed with sodium acetate. Part three of the experiment consists of adding acid or bases into the buffer solution and observing the changes that occur. The independent variable in this experiment is the titration of acetic acid being titrated with sodium hydroxide. While the dependent variable is the pH calculated for the acetic acid as sodium hydroxide

Laboratory 7: Environmental Effects on Enzyme Activity BI-185-03 BY Charlie Kane 10/24/17 Abstract During this experiment we tested the effects that specific environmental conditions have on the enzyme, peroxidase. Through this experiment we developed an understanding of enzymes and their functions. By exposing the enzymes to different environments, we observed a changed in their activity. In order for us to measure these changes we used the technique of spectrophotometry, meaning we used