Thin layer chromatography

Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor, Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45

Chromatography techniques are one of the most useful methods available for the separation or isolation of organic compound from a mixture. The most common used chromatographic technique includes column chromatography, thin -layer chromatography (TLC), etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture. Collecting elutes (run off), and calibrating a curve to show how the column separates molecules by molecular weight, and

TITLE : Reactions and separation of sugars by TLC. AIM : Thin-Layer Chromatography can show many different characteristics of a mixture. It is recognized for isolation , separation ,identification, and anaylsis of the mixture’s components. The purpose of this experiment is to separate carbohydrates into its pure components such as mixtures of monosacrides by TLC. TLC is used to identify sugars in normal and pancreatic disease urine, the procedure is easy and reproducible . INTRODUCTION: technique

Thin Layer Chromatography and Column Chromatography By Maggi Shelton Under the Supervision of Dr. Mills Chavonda The Department of Chemistry and Physics Milledgeville, GA 31061   Abstract: Thin layer chromatography and column chromatography are two different methods that allow for the separation of two miscible solvents. Through column chromatography, a mixture of nonpolar fluorine and polar fluorenone was successfully separated. Thin layer chromatography was then used in order to determine the success

Exploring Thin Layer Chromatography Thin layer chromatography (TLC) is used to to identify organic compounds with common over-the-counter medicine; and the relative strength of the intermolecular forces and how they affect the compound’s polarity within the medications tested. TLC is the physical separation of a mixture into its individual components by distributing the components between a stationary phase, or silica gel; and a mobile phase, or movement of sample up the TLC plate to the solvent

Brianda Mendez Lab 05 Report Introduction: Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. Thin-layer chromatography or TLC, is a solid-liquid form of chromatography, it involves the distribution between two phases. The stationary phase is a polar adsorbent, it is coated on a glass slide or plastic sheet creating a thin layer of the particular stationary phase. The mobile

Thin layer chromatography is the separation of substances using different solvents that run up the TLC plate to find different materials and colors in the sample. Three solvents in this test were one hundred percent acetone, one hundred percent isopropyl, and distilled water. In this test we timed the speed of each solvent and recorded the best color content. PACE Introduction Thin Layer Chromatography is a commonly used experiment in forensics, and is the separation of substances using different

Introduction Thin-layer chromatography, also known as TLC, is a principle that describes how various compounds travel multiple distances when placed as a thin layer on a plate. TLC is a technique that can be used to determine how many components are in a mixture. TLC can also be used to determine a specific compound in a mixture. After performing TLC, the retention factor (Rf) can be used to determine a specific compound in a mixture. The retention factor (Rf) is During TLC, there is a step called

Thin layer chromatography (TLC) is a technique used to identify components within a compound and determine the compounds purity. The purpose of this experiment is to determine what solvent works the best at separating the different pigments of the paprika extract. The solvents used to develop the plate are hexane and methyl tert-butyl ether (MTBE), as well as three mixtures of the two solutions (5% MTBE in hexane, 10% MTBE in hexane, and 30% MTBE in hexane). Hexane is a non-polar alkane that is used

Abstract. Thin Layer Chromatography is a simple procedure that allows you to determine how many and what kind of compounds are in a mixture. By understanding the properties of the TLC system and how it combines with the functional groups of amino acids the retardation factor (Rf) values can be calculated and compared to specific hydropathy values of amino acids. Thin layer chromatography is used to separate and recognize a certain compound. The silica gel that is located on the outside of the TLC