Transfection

The cornerstone of modern medicine is the ability to treat an illness in the absence of a concrete cure. In the case of detrimental diseases such as cancer or HIV, the lack of a cure has not impeded the progress of treatment and prolonging of life. However, there does exist a disease which until recently had no significant treatment: Duchenne Muscular Dystrophy (DMD). Modern gene therapy has provided a chance to cure this illness. DMD is a sex linked disease affecting 1 in 3500 male births. It is

Methods and Materials Mouse PrP would be used for this study since it is known that mouse PrP share a number of structural and biochemical characters with mammalian PrP (Harreist et al. 1991; Gabriel et al. 1992). The materials and methods to be used are similar to those used by Agnes Lau et al. (2015) Mutant PrP plasmid constructs To create new alleles in the PrP which would be equivalent to the copied peptides, to be examined by electron paramagnetic resonance (EPR) spectroscopy– also called electron

thyroid transcription factor 1 (TTF-1), Napsin A, v-raf murine sarcoma viral oncogene homolog B1 (BRAF), v-erb-b2 erythroblastic leukaemia viral oncogene homolog 2 (HER2), c-ros oncogene 1 receptor tyrosine kinase (ROS1), and rearranged during transfection (RET) (4). The EGFR gene mutations seem to be most significant in adenocarcinomas, specifically deletions in Exon 19, and point mutations in Exon 21 (5). Similarly, almost 5% of all NSCLCs are associated with a rearrangement in the ALK

Results Expression of LIMK1 was up-regulated in TCGA breast cancer database The expression of LIMK1 in breast cancer was checked in oncomine website (https://www.oncomine.org/). The TCGA database results (Figure 1) showed that LIMK1 was high expressed in different breast cancer type, including intraductal cribriform breast adenocarcinoma, invasive breast carcinoma, invasive lobular breast carcinoma and mixed lobular and ductal breast carcinoma. These results indicated that LIMK1 could be an oncogene

A nanoparticle is the most basic component in the construction of a nanostructure. In general, the size of a nanoparticle spans the range between 1 and 100 nm. The properties of many conventional materials change when formed from nanoparticles. This is typically because nanoparticles have a greater surface area per weight than larger particles which causes them to be more reactive than other molecules. Self-assembly refers to the process by which nanoparticles or other distinct components spontaneously

“You have competition every day because you set such high standards for yourself that you have to go out every day and live up to that”-(Michael Jordan). This quote perfectly describes my mentality as a child and currently as a young adult. As a child, I was curious and determined as my family recalls. My mother never lets me forget the number of times she had to free my fingers from the holes of shopping carts because I was curious if they would fit. As I got older and a bit more independent, I

Selective breeding Selective breeding: the process by which humans use animal breeding and plant breeding to selectively develop particular traits by choosing which - typically animal or plant males and females - will reproduce and have offspring together. This is the easiest but least effective short term way to select a preferred genotype or phenotype. Humans do this by taking a male and female of a species, each with a desirable trait and breeding them together in the hopes that both desirable

Lab 4: Culturing Escherichia coli involving Cav1.2, Connexin 43, and N-Cadherin By: Zachary Siou EGRB 491 Due: 2/28/17 Introduction Voltage gated channels are necessary components of life processes, in many organisms. One in particular, is the calcium voltage gated ion channel. Often lodged within the phospholipid bilayer, the imbalance of the calcium, or, the inside vs outside concentration, creates a gradient. The channel proteins often undergo conformations, states that which allow or

The company ‘Invivogen’ made a transfectable and simple-to-use plasmid, called psiRNA™, that allows the production of siRNAs within the cells. To transfect means to introduce foreign material into eukaryotic cells. psiRNA is an RNA polymerase III-based plasmid that produces ‘short hairpin’ RNAs (shRNAs). shRNAs are more stable than synthetic siRNAs and since they are continuously expressed within the cells rather than having to be injected into the cytoplasm, this method permits long-lasting silencing

Effect of Thyroid Hormone on Cortical Neuron Dendritic Outgrowth and Cell Viability Thyroid hormones have been known to be essential for normal neurodevelopment for over a century1. A deficiency in thyroid hormone (TH) during gestation, often due to inadequate levels of iodine have serious consequences for offspring including cretinism which is characterized as severe cognitive impairment, ataxia and sensory deficits. Cretinism is the most common preventable cause of brain damage worldwide2. Research