Transfection

Abstract The CRISPR/Cas9 system is an accurate, cheap, and fast way to edit genes. It is used to edit parts of the genome by removing, adding, or altering some sections of the DNA sequence. The CRISPR/Cas9 system was used with HIV cell lines and RNA-directed gene editing to prevent new HIV-1 infections. Both experiments resulted in the stopping of the reactivation of the HIV infection. HIV (Human Immunodeficiency Virus) puts its own genome directly into a host cell during the time

Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique

metabolism, presenting a novel alternative to ineffective treatments. This study investigated a pH sensitive siRNA nanoparticle which circumvents traditional barriers in genetic therapy by promoting intracellular delivery, reducing cytotoxic off-target transfections, and facilitating endosomal escape of siRNA. Acyl-CoA synthetase 5 (ACSL5) was explored as a target for knockdown of glioma viability. Poly(2-(diisopropylamino) ethyl methacrylate) (PDPA) nanoparticles were synthesized via nanoprecipitation, measured

positive charge of this layer allows for favorable electrostatic interactions with negatively charged nucleic acids [155]. The conjugating of NPs with peptides is other design considerations for DNA delivery to the cell nucleus for the successful transfection of genetic material. This is usually accomplished by conjugating peptides that

The authors in this article are trying to better understand how information is processed in the brain, especially the diseased brain. In order to research this information there first needs to be a precise method of spatio-temporal recording of electrical activity in larger neuronal circuits and individual neurons. The authors hypothesized that fluorescent indicators would be useful tools in recording brain activity because they would allow monitoring of a genetically defined neuronal circuit and

A scientist need to demonstrate mammalian cell transfection under very sterile technique since they grow slowly and affected by contamination resulting in lower expression. In addition, the total number of mammalian cell should maintain steady and once there is confluent, cells have no more room to grow

15215) and Luciferase-pcDNA3 (Addgene plasmid 18964) plasmids were purchased from Addgene. 2.2. Cell Culture, Transfection, Reporter Gene And Cell Viability Assays COS-7 and A549 cell lines were maintained at 37°C, 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% (v/v) streptomycin/penicillin (Gibco, Invitrogen). For various transfection and treatment experiments, cells

Genetically encoded fluorescent probe for visualizing glutamate neurotransmission in organotypic hippocampal slices Neurotransmission plays an essential role in communication between neurons. In such process, neurotransmitters are released by the axon terminal and those bind to a receptor on the dendrite of another neuron. Among all neurotransmitters, glutamate is the most significant one in vertebrates. It is used in a large percentage of circuits in the cerebral cortex that support learning and

Specific Aim 2: To determine if Gleevec is effective in inhibiting in vivo cell proliferation in TNBC cells with overexpressed Arg A recombinant plasmid containing a viral promoter driving Arg expression will be inserted into TNBC cells. Another empty recombinant plasmid will be inserted into different TNBC cells. Another plasmid containing a promoter driving Arg expression will be inserted into another group of TNBC cells. Soon after, an immunoblot will be run using these three different TNBC groups’

Second, for the western blotting, the primary antibodies that used to recognize the protein of interest (FLAG-PTEN) are rabbit anti-PTEN antibodies and the secondary antibodies that bind to the primary antibodies and convalently linked substrate to produce visible signal are goat anti-rabbit conjugated HRP (horseradish peroxidase) antibodies; for immunoprecipitation, the antibodies used to immobilize protein of interest are anti-FLAG monoclonal antibodies from morine cell. As introduction above,