Transfection

Results For this experiment our research group produced our own taq. Being able to do this saved our lab a lot of money for such a large-scale experiment. After the moonshine taq was made and amplified using a PCR, we ran a test gel to determine if the taq was functioning and could replicate DNA. Figure 1 shows that the taq was capable of replication. Here we also observe that each band traveled 500 bp, meaning that the mass of the samples was roughly 97ng. We tested both heat-treated taq, and

Project Background (3 pages) Breast Cancer Female breast cancer is the second most common cancer in the world with over 1.6 million cases diagnosed in 2012 (Australian Bureau of Statistics, 2012). Deaths from breast cancer within Australia have increased by 380% from 2003 to 2012 (ABS, 2012)(Smith et al., 2012). An individual’s risk of developing breast cancer increases dramatically with age until 50 where it plateaus giving women a 1:8 lifetime risk (Kamangar et al., 2006)(Davies, 2012). There

Limitless replicative potential is an acquired capability of many cancer cells, and it was listed as one of the original hallmarks of cancer [1]. It is mostly achieved by upregulation of telomerase, a reverse transcriptase DNA synthesis enzyme that maintains the telomeres length. Most tumor cells have a high level of telomerase activity but short telomeres due to prior divisions before achieving telomerase-activating malignancies [2]. In contrast, normal progenitor cells and stem cells have relatively

Yahya Bello To test whether chemokine receptor CXCR3 mediates gene transcription Introduction SAA (Serum Amyloid A) function is similar to a C- reactive protein (an annular pentameric protein that is mostly found in blood plasma and its levels rise in response to inflammation. It considered as an acute-phase protein of hepatic origin, which increases following interleukin-6 secretion by macrophages and T cells) but acts as an acute phase protein, which can be used as an indicative, predictive or

Abnormal Intracellular Zinc Metabolism Caused by Disruption of the TRPL1-TMEM163 Protein Interaction Keywords: Zinc, TRP ion channel, Lysosomes, TMEM163, MLIV Mucolipidosis type IV (MLIV) is a human lysosomal storage disorder characterized early in life by neurodegeneration, visual impairment, low muscle tone, and impaired secretion of gastric acid1. MLIV is caused by loss-of-function mutations or deletions in the Mucolipin-1 gene2 resulting in the absence or a dysfunctional protein product called

I am writing to express my interest in the Research Specialist position in your laboratory in the Department of Microbiology and Immunology. I feel my background as a Bachelor of Science in Molecular and Cellular Biology and my extensive lab experience make me an ideal candidate. During my undergraduate study at the University of Illinois, I worked in a research laboratory for over two years. I completed a senior thesis, earning a Distinction in Molecular and Cellular Biology. I also collaborated

Nematodes were cultured and handled at 20 °C using standard methods (Brenner, 1974). C. elegans strains used in this study are as follows: N2 Bristol and MR156: rrIs01[Pelt-2::GFP; unc-119(+)] X, which were used as wild type. CB3514: lin-23(e1883)/dpy-10(e128) II, YHS47: lin-23(e1883)/dpy-10(e128) II; rrIs01[Pelt-2::GFP + unc-119(+)] X, DH2: zyg-11(b2) II. DS97: mat-2(ax76) II, EU593: mel-26(or184) I, EU640: cul-2(or209) III, UP63: mat-3(ku233) III, NJ582: cul-1(e1756)/unc-69(e587) III, VC141: zif-1(gk117)

Genes are the foundation of life. Biologists throughout history have studied genes with the goal of knowing how they work, what functions specific genes have, and what they can do with this knowledge to further science and society. Techniques involving genes have grown to the level where we can target specific genes to edit them and even alter their function. Some earlier techniques of this include meganucleases, zinc finger nucleases, and transcription activator-like effector nucleases, but each

GOALS The goals of this lab report are to induce E.coli bacteria for producing protein of interest and determined the rate growth and protein concentration after inoculate the E.coli culture. In addition, protein visualizing and analyzing the success of IPTG induction, protein solubility and affinity chromatography demonstrated using SDS-PAGE. This report will also highlight the methodology of affinity chromatography that used to purify fused protein with poly His tag. A comparison between the advantage

Site-directed mutagenesis, also known as oligonucleotide-directed mutagenesis, is a technique that is used to intentionally modify a DNA sequence of a gene by introducing a site-specific mutation. The general method for producing the defined oligonucleotide mutation was developed from a combination of observations on nucleic acids, and it was in the 1978s where enzymatic extension was used as an approach to SDM, using oligonucleotides as primer (Hutchinson, et al., 1978). Since then, this technique