Urease

the endothelium, it causes apoptosis and the related effect of cell desquamation on the endothelium. This happens alongside the secretion of interleukin 6, and 8. Urease is produced, leading to increased risks of pyelonephritis. With the sustained mobility of the Proteus Mirabilis bacteria, the presence of fimbriae and increased urease production leads to increased chances of infection in the upper urinary tract (Zunino, Geymonat, Allen, Preston, Sosa &Maskell,

HELICOBACTER PYLORI Helicobacter pylori have been implicated in cases of chronic gastritis, peptic ulcer diseases( includes duodenal and gastric ulcers). Even though these diseases were linked to stress factors, in 1983, Australian microbiologists Warren and Marshall proved that gastritis and peptic ulcer diseases were infectious diseases caused by a bacterium (1). This bacterium has also been linked to gastric carcinoma and Mucosa associated lymphocyte (MALT) lymphoma (2) This organism was formerly

Citrate-negative cultures would also show no growth in the medium. Urease The urease test was not available, so students asked the lab TA for urease test results for their respective organisms. Results Gram Stain After Gram staining, organism A was classified as a Gram positive organism. On the other hand, organism B was classified as a Gram negative organism

The purpose of this laboratory exercise was to perform tests necessary to be able to distinguish one microorganism from 10 others. Using a series of biochemical tests and characteristics, unknown #22 was concluded to be Pseudomonas aeruginosa. A dichotomous key was mapped out and used during this process. Using this provided guidance as well as organization as to what the result may be. Upon obtaining the unknown organism, it was important to make a streak plate of the bacteria on TSA. The purpose

Abstract Helicobacter pylori is a bacterium responsible for worldwide chronic bacterial infection in human. At least half of the world’s population host H. pylori that causes various gastric pathological outcomes with some leading to gastric cancer. The infection by this bacterium has directly or indirectly caused an impact on economic and general well-being of the people; hence, there is an urgent need to discover diagnostic markers that could be utilized for future development of diagnostic kits

laboratory and clinical settings to accurately determine the identity of the organism of interest. For the identification of the unknown nine different biochemical tests were performed: Triple Sugar Iron Agar , SIM, Methyl Red, Vogues-Porskauer, Citrate, Urease and gelatinase. Microbiology Laboratory Theory and Applications (2009), describes the different Biochemical tests that were performed during this experiment. A brief description of each is provided in the following paragraphs. Triple Sugar Iron

The identification of an unknown bacterium was done by streaking for isolation on a Trypticase Soy Agar (TSA) plate then by following the Gram stain protocol described in “Stain Protocols” by Smith (1). both unknown bacteria were labeled as letter A but then given nickname to describe their morphology on the TSA plate. The specific steps of the gram stain and other test that were used and further information was drawn from the documents “lab 1 microscope, inoculation, isolation” that were provided

The different types of metabolic reactions that were used to identify the Gram-negative unknown were citrate (CIT), gelatin (GEL), hydrogen sulfide (H2S), tryptophan deaminase (TDA), urease (URE) and Voges Proskauer (VP). In the CIT reaction the capability of using citrate as a carbon source for energy is dependent upon the presence of a citrate permease that facilitates the transport of citrate into the cell. Citrate is then acted upon by citrate, producing oxaloacetic acid and acetate that are

Unknown Lab Report April 25th, 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures

Helicobacter Pylori at Tight Junctions Prior to the isolation of Helicobacter pylori (H. pylori} by Robin Warren and Barry Marshall in 1982, it was theorized that peptic ulceration, an ailment first described in 1857 by William Brinton, stemmed from poor diet, tobacco use, and stress due to the failure to definitively identify a causative agent as well as the belief that the inhospitable environment within the human stomach resulted in its sterility (Tanenbaum 2005). Because physicians were ignorant