Urease

(Saphire2, Tecan GmbH, Wien, Austria). The Arg-sensing membranes immobilized with various amounts of arginase and 20 U of urease were used for the measurements of different L-Arg concentrations. The arginase amount for immobilization was optimized with 10 U, 20 U, 30 U, 35 U, and 40 U of arginase and with a fixed amount (20 U) of urease. The immobilization efficiency of two enzymes (urease and arginase) into the Arg-sensing membrane was calculated by dividing the amount

plate is the PEA plate, white showed inhibited growth of the bacteria indicating that the unknown bacteria is gram negative. Table 4 is showing test results for Phenal Red fermentation broths, Methyl Red-Voges Proskauer broths, Catalase, oxidase and Urease. In the three Phenel Red tubes the unknown bacteria #3 tested positive yellow for fermentation. However, when reagents were added in the MR-VP tubes the Methyl Red turned a Pinkish color detecting the unknown bacteria #3 strong acid formed and VP

OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a

stabbed the needle all the way to the bottom of the tube. Then I gently pulled out the needle to the surface of the slant and streaked the surface before removing the needle from the tube. A urease test was next on the agenda. A urease slant is used to indicate whether a bacteria is able to produce urease, that breaks down urea into ammonia and carbon dioxide. This causes the slant to become more alkaline and is indicated by the phenol red within the slant by changing to a hot pink color. Using

ABSTRACT Microbial Induced Calcite Precipitates (MICP) is a new and sustainable technology used to improve the properties of construction materials. This technique works by introducing bacteria solution (e.g., Sporosarcina pasteurii, B. megaterium, Spoloactobacilus, Clostridium and Desulfotomaculum ) into the soil matrix , and then injection of a chemical solution consisting of urea and one of calcium salts (e.g., calcium chloride and calcium acetate ) into the soil matrix several times. A number

utilize the citrate and convert the ammonium phosphate into ammonia and ammonium hydroxide. Urease is an intracellular enzyme that hydrolyzes urea into ammonia and carbon dioxide. Urea broth is used to determine urease production. Phenol red is used as a pH indicator. Urea hydrolysis produces ammonia that raises the pH in the medium and turn pH indicator pink (Leboffe and Pierce). The positive test for urease is pink in color that takes around 8 days. Gelatinase test is done to determine is the microbe

in their patients. In order to isolate different types of bacteria in a patient sample, different tests must be performed. Bacteria can be differentiated based on characteristics such as whether or not they ferment lactose, are motile, or produce urease. It is important to know which tests to run so the minimum amount can be used to identify the causative agent. This helps reduce expenses and yields quicker results so treatment can be administered if necessary. In the case of this identification

a Citrate test, a SIM test, and also a Urease test. The OF glucose test came out positive for a strict fermenter because both tubes turned yellow. The Citrate test came out negative because there was no color change identified. The SIM test showed positive for Sulfur, Indole, and motility. The black precipitate that formed shows the production of H2S and the red color after the Kovács reagent was added indicates Indole

subjected to seven biochemical tests and the results compared to the results of the six known bacteria for the same tests. After performing the Triple Sugar Iron Agar Test, Sulfur Indole Motility Test, Methyl Red Test, Voges-Proskauer Test, Citrate Test, Urease Test, and Gelatin Test it was confirmed that identity of Unknown #15 was Salmonella typhirmurium. While all bacteria retain basic qualities identifying them as prokaryotes they do not function, grow, or even metabolize in the same way. These differences

Escherichia had identical tests results as unknown 65, except for urease and phenylalanine tests (appendix A-16). Also, E. coli is resistant to tetracycline and unknown 65 was not on the antibiogram (4). Unknown also matched Morganella morganii in all performed tests, except for the arabinose and lactose fermentation tests. Bergey’s manual showed the same results. The urease test was positive, and since this test can distinguish urease-positive Proteus, Morganella, Klebsiela, and Providencia from