Urease

The ONPG test also yielded negative results, as the salt stayed transparent instead of turning yellow. The culture grown in the water bath showed the bacteria could grow at 45°C, and the cultures grown on the P-agar plate were larger than 5mm. The urease test resulted in a weak positive outcome on the slant, and a negative result in the bottom of the

The enzyme urease breaks urea down into NH3 and CO2. An orange broth containing urea is used for this test and needs to be inoculated with the gram negative bacteria. A pink color in the medium indicates a urease-positive organism, an orange or yellow is negative. The IMViC test is a series of different tests that differentiate between enterics. One is the Indole

Abstract Microbial Induced Calcite Precipitates (MICP) is a new and sustainable technology used to improve the properties of construction materials. This technique works by introducing bacteria solution (e.g., Sporosarcina pasteurii, B. megaterium, Spoloactobacilus, Clostridium and Desulfotomaculum) into the soil matrix, and then injection of a chemical solution consisting of urea and one of calcium salts (e.g., calcium chloride and calcium acetate) into the soil matrix several times. A number of

recently be identified as the causative agent to gastric ulcers, a condition long believed to be the result of stress and lifestyle habits. The bacteria produce a number of products including urease and vacuolating cytotoxin (VacA). VacA damages endosomal and lysosomal membranes and leads to cell death. Urease helps increase the environmental pH to become less

This result implies that the bacteria are positive for the ability to rapidly convert urea into ammonia and carbon dioxide using the enzyme urease. The indicator phenol red was used which is yellow or orange at any PH below 8.4 and pink or red above 8.4. This explains why the medium was red – ammonia was produced resulting in increased alkanality. Alkaline production increases the pH of the

diplo. Then I determined that I had to focus on tests specific to gram-negative organisms. The tests I chose to perform were: OF glucose test, Citrate test, H2S test, Indole test, Motility test, and Urease test. The OF glucose test concluded that the organism was a fermenter. The citrate, H2S, and Urease tests were all positive. The Indole test was negative. The organism is motile. The unknown organism is Proteus mirabilis.

Name: Mishayla O Moffitt Tube Number: 2 Unknown Organism: Enterobacter aerogenes Introduction: According to the “Microbiology Laboratory Manual,” lab 27 focuses on the family Enterobacteriaceae, this is one of the largest families of bacteria (Wong et al., n.d.). Also all of the members are gram-negative, anaerobe straight rods and can commonly be located in mammal’s intestines (Wong et al., n.d.). All the members Enterobacter aerogenes throughout this report will be referred to as, E. aerogenes

swarming behavior. Swarming occurs when the flagellate rods grow into long chain swarmer cells, the swarmers gather together forming “rafts” that swim together, growing into a complex biofilm. P. mirabilis virulence factors include swarming motility and urease production. Ref: Joan L, John F. microbiology: an evolving science New York 2009, Belas, Schneider and Melch. Journal of bacteriology Dec. 1998 p. 6126-6139. Its scientific

Autumn White Biology 258-05 November 26, 2012 Unknown #19 Enterbacteriaceae Enterobacter aerogenes ABSTRACT The objective of this report was to identify an unknown microorganism through several differential media tests. Over the course of a couple weeks, ten tests were performed. First, a gram stain was performed, indicating the bacterium was gram negative. An aerotolerance test determined that the bacterium was a facultative anaerobe. Next, a negative result in the

After performing the gram stains, I needed to outline a plan of action for my metabolic testing. I employed the help of a dichotomous key, which allows you to make successive choices between two alternatives. This key would provide a clear cut path for my metabolic testing in order for me to successfully identify my two unknown bacteria. First, I divided the key into gram positive and gram negative bacteria. Then I divided the positive half of my key by morphology, rods and cocci. The other negative