Urease

You did a wonderful job on your PowerPoint presentation! I see that a serum H pylori test was done and came back positive for the patient. The test has a high specificity and sensitivity, and it is useful for detecting a newly infected patient. As providers, it will important to know that the serum H pylori test is not a good test for follow-up of treated patients because the results do not indicate a present infection with H pylori due to the fact that the antibody titer can remain elevated for

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acetoin which tells you that the organism is a butanediol fermentor. For the Urease test, I incoluated my Urea test tube with my unkown bacteria from a TSA plate using and inoculating loop. The Urea tube was then incubated at 37 degrees Celsius for 8 days to observe for a color change. The Urea tests for the ability of a bacteria grown in urea broth produces urease. This medium contains the pH indicator phenol red. If urease is produced the pH of the media will raise thus causing the phenol red to change

gram negative. The appropriate identifying tests for this type of bacteria were performed, including the use of SIM agar, Urease, Citrate and OF Glucose tests. Once inoculated, these tests are incubated at 37 degrees Celsius for 24-48 hours. The findings of these tests revealed that the organism is motile, positive for indole production, negative for sulfur, negative for urease, negative for citrate and is a fermenter. The characteristics displayed are indicative of Escherichia coli. These tests

in different mediums. In this experiment, pH indicators, colour indicators, metallic ions, and Kovac’s reagent all aide in the differentiation of different bacteria under different conditions. Proteus vulgaris for example is a rapid production of urease and this is shown through the phenol red indicator, turning from yellow to pink. This experiment is usually done to distinguish from Proteus and other bacteria, which helps to isolate these bacteria since they are infectious (O'hara, et al., 2000)

Section I- Introduction Microbiology is the biological study of organisms that are too small to be seen with the naked eye (1). Microbiology includes examining a variety of different microbes such as bacteria, archaea, protozoa, fungi and viruses (1). By examining these microbes, their relationship with people and the environment they grow in, is better understood. These examinations of microbes have played a major role in comprehending how microbes can be helpful and harmful, and how

Tests used in the species elimination tree were cytochrome C, sucrose, mannitol and lactose fermentation, acetoin production, and urease. Species that were 11-89% positive for a test were not eliminated. The unknown organism was negative for cytochrome C, which eliminated the cytochrome C positive S. caseolyticus, S. sciuri, and S. lentus. The organism was positive for sucrose fermentation

was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes. Introduction Gram negative and gram

Introduction Medical microbiology is an aspect of microbiology sciences. Microbiological systems play a vital role in people’s everyday life. Specifically, bacteria maintain processes within and on the body. Bacteria can also cause disease from an infection of the opportunistic bacteria living symbiotically with people or foreign bacteria introduced from various plausible sources. The identification of the unknown bacteria was accomplished by an independent study of numerous biochemical tests. The

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium