Urease

each compartment with an unknown bacteria, a reaction will occur in 18-24hours. The results were organized in a Reading Table and given a number based on the results. With this number it is possible to identify the bacteria. Urease test (URE): The substrate is the urea and urease is an enzyme that catalyses the hydrolysis of urea into carbon dioxide and ammonia. The function is to increase the pH of the environment due to the production of ammonia.

Introduction: An organism of unknown origin was selected by the course instructor in an effort to properly identify the organism with the utilization of a series of biochemical tests. This exercise was of extreme importance during the termination of the course because it was essential that each student be able to apply the practical knowledge learned through class lectures, and lab practicals in an effort to individualize such experience so that each student could properly handle a bacterial culture

4.1 Crop yield and juice quality Soil sickness of sugarcane monoculture, expressed as a reduction of cane yield, stalk diameter and biomass when sugarcane is cultivated on the same soil sequentially, is one of the foremost problems in sugarcane production, particularly in field conditions (Li et al. 2015; Li and Yang, 2015). Sugarcane is an important crop in Guangxi, China, but due to its long duration annual economic returns tend to be lower than some other crops. Intercropping of short duration

Ashley Whitehead BIOL1134K-01 Dr. Balding 10 November 2016 Identification of Unknown Bacteria Number 63 The best and most accurate way of identifying an unknown microorganism is by sequencing its DNA, but this is very expensive and only used in highly qualified labs. So, the identification of unknown bacteria number 63 was be done by putting the bacteria through numerous laboratory tests. Microorganisms are different among each other by their macroscopic morphology, microscopic morphology, and

Introduction The objective of this experiment is to identify the organisms of two unknown bacterial cultures. Students must identify the species of the unknown bacteria by utilizing the techniques and information learned in previous laboratory exercises. These techniques include streaking for isolation, Gram staining, and specific biochemical tests. Students are given a map known as a dichotomous key, a guide in determining the identity of their unknown sample. Identifying microbes using a series

TSI Acid but,acid slant,gas production and no H2S Acid but, acid slant, no H2S and no gas formation Acid but,alkaline slant,no H2S and no gas Catalase Positive Positive Positive Indole Positive Negative Negative Oxidase Negative Negative Positive Urease Negative Positive DISCUSSION AND RESULTS Organism A - E.coli Organism B -S.Aureus Organism C - P.Aeroginosa Staphylococcus aureus is a gram positive coccus found in the human respiratory tract and on the skin. It is a facultative anaerobic organism

The Christensen’s urea agar slant utilized a phenol red indicator that changed colors to pink when pH conditions became basic. If urease were present, it would hydrolyze urea to ammonia and CO2 (17). Because no color change was observed, the organism was negative for urease. However, the organism was positive for catalase, which was indicated by the formation of bubbles following the addition of H2O2 to the TSA slant. This showed the breakdown of H2O2

produced a black butt and red slant indicating glucose fermentation and confirmed sulfur gas production, leaving only Proteus vulgaris as another possible outcome. Casein, gelatin, and urease testing were needed to conclude my results, as only P. vulgaris would be found to possess the enzymes casease, gelatinase, and urease, which, none of those were found in my unknown organism. Both of the two organisms were negative to hydrolyze starch, and neither can metabolize oxygen. Most infections with Salmonella

5. Detection methods There will be a high probability of positive serology or another test when using the test-and-treat strategy in populations with high prevalence of H. pylori regardless of symptomatology (24). Hp laboratory diagnostic methods are divided into two categories. One is invasive test: it is through the endoscope to obtain gastric mucosal tissue as a test material, Conventional endoscopic exam is usually performed to diagnose H. pylori-associated diseases。Culturing of H. pylori from

Unknown 12 was proved to be Proteus vulgaris. This organism was a gram-negative rod growing in single. This organism, which grew on a TSA plate, was catalase positive. A drop of H2O2 was added on to the colony and it began to bubble. Therefore, the catalase enzyme was present and broke down the peroxides in to non-toxic forms H2O and O2. Unknown 12 was oxidase negative. Three drops of Kovac’s reagent was added to a colony growing on the edge of the plate. The enzyme oxidase, reacts with oxygen and