Western blot

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  • Western Blot Analysis Essay

    1181 Words  | 5 Pages

    Immunoprecipitation and western blot analysis LV-FLAG-HA-T2A-GFP and LV-hZNF804A-FLAG-HA-T2A-GFP constructs were transfected into HEK293 cells with N-terminal V5-tagged APEX1, EIF3K, FEZ1, LGALS1, PDHB, PDZD4, and RPSA overexpression constructs from DNASU. 72 h after the transfection, cells were lysed with cell lysis buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0) and collected by centrifugation at 14 kg at 4°C for 15 min. 3 mg of lysate protein was incubated

  • Beta-Galactosidase and Western Blot

    4114 Words  | 17 Pages

    Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes, namely, _lacZ_, _lacY_ and _lacA_, the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_, _lacY_ and _lacI_ would code for beta-galactosidase, galactosidase permease

  • Western Blot Analysis

    275 Words  | 2 Pages

    The technique used in this experiment was western blot to determine the protein levels in different cow’s stages; fetal calf serum, newborn calf serum and cow serum. Western blot is technique commonly used to identify proteins by its movement in the gel electrophoresis. Western blot is use to separate protein based on its molecular weight in gel electrophoresis. The proteins separated in the gel, then transferred to a nitrocellulose membrane using an electron current (1). Finally the membrane is

  • The Effect Of Protein And How They Interact And Function Within Organisms Essay

    2373 Words  | 10 Pages

    Proteomics is the study of protein and how they interact and function within organisms. This results in catalyzing and cataloguing proteins; while comparing how each variation of protein expression in different conditions. This is done in a large scale manner; a good example of this is the Human Genome Project that took place from 1990 to 2003. With the field of Proteomics it has given the ability to understand genes and to comprehend why there’s so much diversity of protein, including their importance

  • A Research Study On Cultured And Handled At 20. C Using Standard Methods ( Brenner ) Essay

    1268 Words  | 6 Pages

    Nematodes were cultured and handled at 20 °C using standard methods (Brenner, 1974). C. elegans strains used in this study are as follows: N2 Bristol and MR156: rrIs01[Pelt-2::GFP; unc-119(+)] X, which were used as wild type. CB3514: lin-23(e1883)/dpy-10(e128) II, YHS47: lin-23(e1883)/dpy-10(e128) II; rrIs01[Pelt-2::GFP + unc-119(+)] X, DH2: zyg-11(b2) II. DS97: mat-2(ax76) II, EU593: mel-26(or184) I, EU640: cul-2(or209) III, UP63: mat-3(ku233) III, NJ582: cul-1(e1756)/unc-69(e587) III, VC141: zif-1(gk117)

  • Purified Protein Lab Report

    983 Words  | 4 Pages

    to localize the expression of a gene or its product (Northern, western, in situ hybridization, immunofluorescence, etc.), to determine the structure of the protein (NMR, crystallography ...) or to find partners proteins (double hybrid, immunoprecipitation ...) give important information on the function of a gene (V Ecochard -2011). This experiment will focus on SDS PAGE of purified protein and cell lysate samples, Western blot analysis procedure, interpretation of purified protein and lysate

  • Functions Of Eukaryotic Cells For Various Functions And Processes

    1548 Words  | 7 Pages

    Microtubules are an essential part of eukaryotic cells for various functions and processes, and are located throughout the cytoplasm in the cytoskeleton. Microtubules are essential for multiple cellular processes such as, cellular division and cellular movement and transport(6). Since microtubules are required for many cellular processes, mutations involving microtubules can cause various diseases including neurodegenerative diseases and cancer(5). One of the most common neurodegenerative diseases

  • A Research Study On Jane Doe Essay

    923 Words  | 4 Pages

    decreased appetite, and losing weight, fever and complained of a sore throat with white patches developing. Her neck lymph nodes were also showing signs of swelling. Her Physician immediately ordered an Enzyme-Linked Immunosorbent (ELISA) test, Western Blot testing, CD4 T-cell count, Complete Blood Count (CBC), Erythrocyte Sedimentation Rate (ESR), and a p24 Antigen test and was asked to come follow up when the results where in. ________________________________________ ASSESSMENT Upon Jane’s follow

  • Quiz Questions On The Properties Of A Protein At Dna Level

    1668 Words  | 7 Pages

    SDS-Polyacrylamide Gel electrophoresis 2. b) Change the properties of a protein at DNA level 3. c) Nuclear magnetic resonance 4. b) Northern blot hybridization 5. Far Western Blot analysis and Protein arrays Far Western Blot analysis: Far Western Blot analysis is the technique mostly resembles Western Blot analysis, but differs in way that proteins are separated on a blot. Biotinylated non-antibody proteins are used for immobilizing the protein of interest, which are then overlaid with an antibody with

  • The Importance Of Animal Protocol

    831 Words  | 4 Pages

    for harvesting tissues preparation. The animals were sacrificed at 72 h after CA/CPR. A left brain was used for ROS detection, TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining and immunostaining. The right hippocampal brains used for Western blot. ROS detection Harvested brains were embedded and frozen in CM1950 Cryostat Microtome (made in German Leica), ROS production in neurons was assessed with dihydroethidium (DHE) as an indicator.Serial, coronal frozen-sections (5µm thick) were incubated