Western blot

SDS-Polyacrylamide Gel electrophoresis 2. b) Change the properties of a protein at DNA level 3. c) Nuclear magnetic resonance 4. b) Northern blot hybridization 5. Far Western Blot analysis and Protein arrays Far Western Blot analysis: Far Western Blot analysis is the technique mostly resembles Western Blot analysis, but differs in way that proteins are separated on a blot. Biotinylated non-antibody proteins are used for immobilizing the protein of interest, which are then overlaid with an antibody with

for harvesting tissues preparation. The animals were sacrificed at 72 h after CA/CPR. A left brain was used for ROS detection, TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining and immunostaining. The right hippocampal brains used for Western blot. ROS detection Harvested brains were embedded and frozen in CM1950 Cryostat Microtome (made in German Leica), ROS production in neurons was assessed with dihydroethidium (DHE) as an indicator.Serial, coronal frozen-sections (5µm thick) were incubated

Resveratrol Attenuates Neuronal Apoptosis after Subarachnoid Hemorrhage via inhibiting the expression of TXNIP in Rats Abstract Objective: Subarachnoid hemorrhage (SAH) is a fatal condition with a few available effective treatments. Resveratrol (RES), a natural polyphenolic compound, has a powerful anti-apoptosis property. In addition, its neuroprotective effects has been exhibited in neurodegenerative diseases, ischemic brain damage, and SAH. However, the underlying molecular mechanism of RES in

Synopsis of “An evidence in vitro for the influence of bisphenol A on uterine leiomyoma” 1. Introduction Uterine fibroids are the most common noncancerous growths of the uterus also known as leiomyomas or myomas. They are not usually associated with an increased risk of uterine cancer and almost never develop into cancer but are considered as a common cause of hysterectomy. Gynecological researchers consider that the increase in the incidence of this type of tumor is related to the presence of

Guy11 was used as wild type of M. oryzae for transformation in this study. Mycelia harvested from liquid CM was used for DNA and RNA extractions. For vegetative growth, small squares of mycelia were cut from the edge of 7-day-old strains and placed onto the media (CM, MM, OM and SDC) and incubated in the dark at 28°C for another 7 days. For conidia collection, mycelial blocks were inoculated on SDC (100 g of straw, 40 g of corn powder, 15 g of a gar in 1 L of distilled water) agar media [50] maintain

the other actors. The man in front of me followed the script by justifying why he disregarded his symptoms. A sudden, yet terrifying thought caged my mind. Without thinking, I charge out the studio and to the chemist across the road. One AIDS Western Blot Blood Test Kit was purchased, and I fix my movement on traveling to the nearest restroom. While panting for air, my trembling hands unpack the cardboard box on the communal basin. Without thinking I pierce the needle through my dry skin. The reflection

Meaningful Use in the medical field describes the adaptation of health care in three stages and is based on things like improving quality and efficiency of patient care, engaging patients and family members in the patients’ health, and the improvement upon population and public health. Stage one, which began in 2010, focused on the use of Electronic Health Records. The second, which took root in late 2012, increased the implementation of patient education, participation, and care-coordination. The

The Pros of Mandatory HIV Testing and Disclosure of HIV Status The universal precautions of the Centers for Disease Control do not eradicate all risk to the patient or health care provider, says Baillie et al. (p. 129). While health care providers in all institutions have been educated in universal precautions, Beck, a registered nurse, cautions that some employees have failed to comply with the recommended procedures from the Centers of Disease Control. Some nurses find goggles, gloves, and

The growth of HIV cases in Indonesia is one of the most rapid ones among the countries in Asia (UNAIDS 2013). Indonesia’s Ministry of Health estimates that more than 500.000 people in Indonesia will become infected with HIV in 2014 unless there is an acceleration of HIV prevention programs (UNICEF Indonesia 2012). In Indonesia, the testing of HIV currently is done in voluntary counseling and testing (VCT) clinics in selected hospitals. The patients are required to visit the clinic to do the testing

Goffman (1963) defined stigma as any condition, attribute, trait, or behavior that is deeply discrediting and reduces the bearer from a whole and usual person to a tainted and discounted one. . Stigma consist of an attributes that marks people as different leading to devaluation. Stigma is socially constructed (Major & O’Brien, 2005) thus is dependent both on relationship and context. In fact, Goffman (1963) emphasized using the “language of relationships” (p. 3) when considering stigma. A subsequent