Week 7 laboratory reflection - Plant Transpiration

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Indiana University, Bloomington *

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Chemistry

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Dec 6, 2023

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Name: Laboratory Reflection: Plant Transpiration (Part 2) Instructions: Make sure to scroll through the entire document to answer all questions. It would also help with grading if you put your typed answers in a different (but readable) color font . Save and upload your completed assignment to the associated assignment page on Canvas. It is the student’s responsibility to confirm that the correct file was submitted to Canvas by the due date. 1. (.5pts) Transfer the data you collected on page 6 of your laboratory protocol packet to the tables below (type in your values). Vial # Treatment Initial mass (g) Final mass (g) Change in mass (g) Transpiration rate (g/day) 1 Sun 58.673g 33.123g 25.550g 3.65g/day 2 Sun 59.387g 49.321g 10.066g 1.438g/day 3 Sun 60.702g 31.713g 28.989g 1.141 g/day 4 Sun 58.515g 45.661g 12.854g 1.836 g/day 5 Sun 60.999g 58.526g 2.473g 0.353 g/day 6 Sun 60.387g 55.865g 4.522g 0.646 g/day 7 Sun 58.015g 55.463g 2.522g 0.365 g/day 8 Sun 60.539g 58.434g 2.105g 0.301 g/day 2. (4pts) Create a graph that accurately illustrates the results from your plant transpiration experiment. Your graph must include all relevant elements, including a figure caption. Your 1

caption should describe the graph, include a brief description of the experimental design (~1 sentence), and a results statement that includes your statistical findings (see hint below on how to do this). If needed, see additional resources linked within the assignment page on canvas. [Insert your graph and caption here] Transpiration Rates of Corn Red: Exposed to Sunlight Green: Control (No Sunlight) There was a significant difference in the transpiration rates (g/day) between plants that were exposed to sunlight and those that were not (t=3.5136, df=6, p=0.0126). 3. (2pts) What do your experimental results tell you about transpiration rates in corn? In other words, what do your findings suggest regarding the impact of the environmental condition 2 Note on figure captions: A complete figure contains both an image and a caption. Together, these two parts should provide enough information that a reader can understand the data presented without referring to the text. A figure caption should contain a concise description of the graphic allowing a reader to understand the figure.

you tried to manipulate? Explain and be specific. (Hint: think back to your original research question). These results tell me that corn transpires at a much higher rate per day when exposed to sunlight. When all other variables, such as wind, temperature, and access to water and nutrients are controlled for, the sunlight makes a quantifiable difference. 4. (2pts) If your findings did not support your initial hypothesis and predictions, describe why that might be the case, and what you would do differently if you were to repeat this experiment. If your findings did support your initial hypothesis and predictions, describe another way that you could go about testing your hypothesis to provide additional confidence in your findings and interpretations. I think another way to test to add confidence would be to do the experiment for 3 days, record the results, and then switch the plants to test how much of the further transpiration occurs due to an increased leaf size when growing during the experiment, as the plants exposed to sunlight were significantly bigger than the ones in the shade. 5. (1.5pts) What are two other environmental conditions that you would expect to influence transpiration rates and in what way? Explain your reasoning/logic for each. Transpiration rates would probably be influenced by humidity because the air being wet already would mean that it wouldn’t draw as much out as dryer air. Transpiration would also likely be affected by soil depth in a less controlled environment. More rain water would drain down, allowing for less absorption by roots and therefore less water to be transpired. 3

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