10. Here is a graph describing the kinetics of PCR. Answer the questions below.PCR Product (Log 2)CBAPCR Cycle Numberi. Reaction is in what phase when it isat point A in the graph?ii. Reaction is in what phase when it isat point B in the graph?iii. Reaction is in what phase when it isat point C in the graph?Name of Phase:11.✓ ✓ ✓ DNADNA was isolated, diluted 1:100 in water, and an optical density (OD) of 0.12 was measured at260nm." What is the concentration of the DNA solution if you assume that an optical density of 1.0 OD 260 is(2.equivalent to 50ng/μl?12. (stock solution has a concentration of 3.5 X 10° target DNA molecules/al..a. If you make a 1:100 dilution of this stock, what is the resulting concentration of the DNA?b. If you add 5 μl of the diluted DNA in part a (above) to a PCR reaction tube. How many target DNAmolecules did start with in the PCR reaction tube?4

Key references:Why is Real-time PCR is superior to end-point PCR? (n.d.).…

Answered: 10. Here is a graph describing the…

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 6SQ

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1. Why is each of the following reagents required to be in the PCR reaction tube? Primers, Taq DNA polymerase, nucleotide mix, reaction buffer 2. Imagine that you forgot to add nucleotides to your PCR master mix. How would this affect the outcome of your PCR reactions? a) If you made the mistake described in the previous question, which control would give you unexpected results? (In other words, if you forgot to add nucleotides, which control reaction tube would have results that are different that they would be if you had prepared them correctly?) 3. If you found that there was DNA amplification in your negative control tube, what could be an explanation for that result?
Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The appropriate modification for the next PCR reaction is to: a) increase the concentration of Taq polymerase b) increase the number of PCR cycles c) decrease the template denaturation temperature d) reduce the concentration of primers
Polymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes. In 150 words or fewer, describe 1.) the different stages of a single PCR cycle, 2.) how many cycles of PCR your reactions will undergo, and 3.) why multiple cycles are necessary.
1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.
1. What are some reasons to do PCR? 2. Why do we use a special DNA polymerase from thermophilic bacterium? 3. What are the three temperature ranges used for PCR and what is happening at each step? 4. Why is PCR very susceptible to contamination? all 4 questions please, thanks!
6. Which of the following steps are catalyzed by Taq DNA polymerase in a PCR reaction?a. Denaturation of template DNAb. Annealing of primers to template DNAc. Extension of primer end on the template DNAd. All of the above Please also explain the answer properly and refer to NCERT XII (chapter 11) textbook.
Choose the correct statements from the list below. There may be more than one correct statement. A) If you start with 2 DNA templates, after four rounds of PCR you'll have 32 copies B) PCR is useful in making millions or billions of copies of a gene so that it is present in a quantity large enough to study C) quantitative PCR is very similar to PCR, but fluorescent probes are added so that we can measure how much PCR product exists by examining how much the reaction fluoresces D) In real-time reverse transcriptase PCR, the RNA is used as a template to make a cDNA copy (through reverse transcriptase)
Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified Organism
1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uL
Please answer these two questions regarding PCR a) First question you must use the image: What is the DNA profile for suspect B for the TH01 locus? b) What determines the target sequence amplified in PCR?
1: what actually happens in lab for COVID PCR. 2: How PCR for COVID testing is done from start to finish. 3: explain how PCR is done to detect COVID from patients. 4: how/what is done for COVID PCR.
All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚C

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