5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts 6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above 7. The temperature to incubate the bacteria in the above mentioned experiment should be __: a) 22 oC b) 37 oC c) 98 oC d) 37 o

1. What is the purpose of an autoclave and what is its mechanism?2. Why is there a need for an incubator in the lab? How about incubating cultures at roomtemperature? How can this affect microbes?3. What are the different hoods in the lab and why are they important?4. How does the light compound microscope magnify an object? Outline the process5. Explain why an inverted image is seen under a compound microscope.6. What is the purpose of the oil when using oil immersion objective?7. Why should the microscope be calibrated for each objective and prior to each use?8. When carrying the microscope, why should one hand hold the bottom of the base?

In your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?

In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?