A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction The researchers begin to characterize the enzyme. a)In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 μM s-1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) b)In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μM, the researchers find that V0300 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) c)Further research shows that the purified happyase* used in the first two experiments wasactually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation, and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μM s-1, and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of αand α’
A research group discovers a new version of happyase, which they call happyase*, that catalyzes the
a)In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 μM s-1. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.)
b)In another experiment, with [Et] at 1 nM and [HAPPY] at 30 μM, the researchers find that V0300 nM s-1. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.)
c)Further research shows that the purified happyase* used in the first two experiments wasactually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation, and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 μM s-1, and the measured Km in (b) is now 15 μM. For the inhibitor ANGER, calculate the values of αand α’.
![НАРPY — SAD](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fec4da29f-da07-46c2-adcc-3d11b325511e%2F04e7e067-5d56-4e49-bbd2-8ce967cb9777%2Fjdrcxis_processed.png&w=3840&q=75)
![](/static/compass_v2/shared-icons/check-mark.png)
Trending now
This is a popular solution!
Step by step
Solved in 2 steps with 2 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
![General Chemistry - Standalone book (MindTap Cour…](https://www.bartleby.com/isbn_cover_images/9781305580343/9781305580343_smallCoverImage.gif)
![Living By Chemistry: First Edition Textbook](https://www.bartleby.com/isbn_cover_images/9781559539418/9781559539418_smallCoverImage.gif)
![General Chemistry - Standalone book (MindTap Cour…](https://www.bartleby.com/isbn_cover_images/9781305580343/9781305580343_smallCoverImage.gif)
![Living By Chemistry: First Edition Textbook](https://www.bartleby.com/isbn_cover_images/9781559539418/9781559539418_smallCoverImage.gif)