A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. a. What is the expected result if the protein stays permanently in the nucleus? b. What is the expected result if it is a shuttling protein? Explain your answer.

A protein was recently discovered to be located in the nucleus. However, it is uncertain whether this is a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time in the nucleus. One way to resolve this question is to perform the following steps: • Fuse the cells expressing the fluorescently labeled protein in their nucleus with the cells that are not expressing it. • Treat the cells with an inhibitor of protein synthesis to prevent the synthesis of more labeled proteins. • Observe for fluorescence in the fused cell with two nuclei. a. What is the expected result if the protein stays permanently in the nucleus? b. What is the expected result if it is a shuttling protein? Explain your answer.

Name: ANSWER QUESTION A AND B. A protein was recently discovered to be locat
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Description: ANSWER QUESTION A AND B. A protein was recently discovered to be located in the nucleus. However, it is uncertain whether thisis a protein that permanently stays in the nucleus or a shuttling protein that stays most of the time inthe nucleus. One way

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter10: Cell Division And Mitosis

Section: Chapter Questions

Problem 1ITD: Biologists have long been interested in the effects of radiation on cells. In one experiment,...

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Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.
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