Association for the Advancement of Science. Reprinted with permission from AAAS. FIGURE 6.13 16- 16 15- 15 The measured force versus distance curve is shown for the P5abcAA form of RNA. The black trace represents the stretching of the molecule, and the red curve repre- sents the refolding of the molecule as the beads are brought back to their original separation. The left panel shows data for an increase in force of 1 pN s¹, and the right panel shows the corresponding results for 10 pN s™¹. 14- 14 13- 13 12 12 Source: Figures 1 and 2 from "Reversible Unfolding of Single RNA Molecules by Mechanical Force" by Jan Liphardt, et al., in Science 27, Vol. 292 No. 5517: 733-737, April 2001. Copyright © 2001, The American Association for the Advancement of Science. Reprinted with permission from AAAS. 10 pN s-1 1 pN s-1 The paper shows force vs distance curves of RNA unfolding and refolding (reproduced above). What is the difference between these two curves? In nature, do you think that the forces acting or molecules would yield a graph like the one on the left, or on the right? Explain why. 220 Extension (nm) 240 260 280 220 Extension (nm) 240 260 280

Association for the Advancement of Science. Reprinted with permission from AAAS. FIGURE 6.13 16- 16 15- 15 The measured force versus distance curve is shown for the P5abcAA form of RNA. The black trace represents the stretching of the molecule, and the red curve repre- sents the refolding of the molecule as the beads are brought back to their original separation. The left panel shows data for an increase in force of 1 pN s¹, and the right panel shows the corresponding results for 10 pN s™¹. 14- 14 13- 13 12 12 Source: Figures 1 and 2 from "Reversible Unfolding of Single RNA Molecules by Mechanical Force" by Jan Liphardt, et al., in Science 27, Vol. 292 No. 5517: 733-737, April 2001. Copyright © 2001, The American Association for the Advancement of Science. Reprinted with permission from AAAS. 10 pN s-1 1 pN s-1 The paper shows force vs distance curves of RNA unfolding and refolding (reproduced above). What is the difference between these two curves? In nature, do you think that the forces acting or molecules would yield a graph like the one on the left, or on the right? Explain why. 220 Extension (nm) 240 260 280 220 Extension (nm) 240 260 280

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter29: Transcription And The Regulation Of Gene Expression

Section: Chapter Questions

Problem 16P

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