b) What glassware and lab equipment would you need? 1. This question is adapted from Harris 19-D.Carnosine is a dipeptide whose antioxidant properties protect cells from free radicals. Carnosine wasdetermined by derivatization with naphthalene-2,3-dicarboxyaldehyde and cyanide followed byfluorescence detection using excitation at 445 nm and emission at 490. Quantification was by standardaddition. To four aliquots of 20-ul cell lysate were added volumes of 100µM carnosine standard togenerate final concentrations of 0, 1.0, 2.5, and 5.0 µM added carnosine. Solutions were diluted to 70µlbefore addition of 15µl of 5 mM naphthalene-2,3-dicarboxyaldehyde and 15µl of 10 mM NaCN.HH2N.OHCarnosineHN.Naphthalene-2,3-dicarboxyaldehydeCyanideOHFluorescent productHN.Fluorescence intensityConcentration (uM) of added carnosinein final 100 µL0.00.4651.00.6982.51.0295.01.651

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b) What glassware and lab equipment would you need?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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