Capping of eukaryotic mRNA occurs at the 5' end O occurs at the 3' end O occurs at both ends O doesn't occur at all
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- Post-transcriptional Modification of Eukaryotic mRNAs What might be the advantages of capping, methylation, and polyadenylylation of eukaryotic mRNAs?The Lariat Intermediate in RNA Splicing Draw the structures that comprise the lariat branch point formed during mRNA splicing: the invariant A. its 5’-R neighbor, its 3’-Y neighbor, and its 2’-G neighbor.Comparison of Prokaryotic and Eukaryotic Transcription Make a list of the ways that transcription in eukaryotes differs from transcription in prokaryotes.
- A pre-initation complex (PIC) is required for transcription to begin. State which TFII protein has catalytic function......... cen not be true about in ditu hybridization. Use to reveal where gene expressed at mrna lvel. perform in none live animal/ tissues. reveal whether 2 genes are active in same cell. determine the function of gene⦁ Following is an mRNA sequence reported in data base.5’ ACC AGA ATG ACC ATG GCA 3’ ⦁ There are two ATG’s. From which the translation can be initiated.⦁ 1st ATG⦁ 2nd ATG⦁ None⦁ If there are multiple possible start codons, how can you identify the original start codon? Explain.Your Answer: ⦁ This is an mRNA sequence, but why there are T’s instead of U’s? Explain.Your Answer:
- Write all the possible mRNA sequences that can code for a simple tripeptide segment Leu-Trp-Glu. Your answer will give you some idea of the number of possible mRNAs that can code for 1 polypeptide. Hint: there are 12 such sequencesmRNA maturation in eukaryotes includes all of the following EXCEPT: A. Topoisomerase activity B. Endonuclease C. 5’ 7’methylguanosine D. Polyadenylation E. snRNP function⦁ Following is an mRNA sequence reported in data base.5’ ACC AGA ATG ACC ATG GCA 3’ If there are multiple possible start codons, how can you identify the original start codon? Explain.Your Answer:
- The method of Northern blotting is used to determine the amountand size of a particular RNA transcribed in a given cell type.Alternative splicing (discussed in Chapter 14) produces mRNAsof different lengths from the same gene. The Northern blot shownhere was obtained using a DNA probe that is complementary tothe mRNA encoded by a particular gene. The mRNA in lanes 1through 4 was isolated from different cell types, and equal amountsof total cellular mRNA were added to each lane. Explain these results.Researchers investigated whether time of day affects the rate at which certain mRNAs produced by plants are broken down. At either 1 hour after the start of the light period (morning) or 8 hours after the start of the light period (afternoon), the researchers treated identical plant seedlings with a compound that blocks transcription (time = 0 min). The researchers measured the percent remaining of two mRNAs, mRNA G and mRNA H, over the course of 120 minutes. The data are shown in Figure 1. Based on the data, which of the following best describes the relationship between light and the degradation of mRNA G and mRNA H? a. mRNA G and mRNA H degrade at the same rate during morning exposure to light. b. An increase in exposure to light causes an increase in the degradation of both mRNA G and mRNA H. c. Exposure to more-intense afternoon light causes both mRNA G and mRNA H to degrade more rapidly in the afternoon…49Eukaryotic RNA polymerases can be distinguished by their sensitivities to ( ). 50Each bacterial mRNA open reading frame has its own ( ) codon. 51Two nuclear splicing intermediates resemble ______________. A.helicesb.lariatsc.hairpinsD.projections