Describe precisely the catalytic activity of DNA polymerase I that removes RNA primers. O 3' to 5' exonuclease 5' to 3' exonuclease O 5' to 3' endonuclease O 3' to 5' endonuclease
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- The epsilon subunit of DNA polymerase III is responsible for its _______ activity.A-5'---->3' polymerase B- Sliding clampC-3'----->5' exonuclease activity D-5'------>3' exonuclease activityDuring high stress environments, it has been found that some bacteria activate a genetic mechanism that allows them to incorporate more mutations into the DNA during replication. Would the following two enzymes be impacted by such a mechanism? (i)DNA polymerase IIIii) HelicaseAn RNA-dependent DNA polymerase that carries the RNA template with it to synthesize repeats at the 3'-ends of chromosomes is called: telomerase topoisomerase DNA ligase DNA polymerase γ (gamma).
- Describe an experimental approach to determining the processivity of a DNA polymerase (i.e., the number of nucleotides incorporated per chain per polymerase binding event).In eukaryotes, RNA primers are primarily removed by a. DNA polymerase I. b. DNA polymerase α. c. flap endonuclease. d. helicaseIn one, simple sentence define the function of the following 1. Helicase = 2. Alpha subunit of DNA polymerase III =
- Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?a) 5’ to 3’ exonuclease activityb) 3’ to 5’ exonuclease activityc) 3’ to 5’ endonuclease activityd) 5’ to 3’ endonuclease activityEukaryotic DNA polymerases do not have 5'-3' exonuclease activity. _______ removes primers in eukaryotic DNA replication. DNA polymerase V Proteinase K RNase H PrimaseThe linking of the 5’ end of one Okazaki fragment with the 3’ end of an adjacent Okazaki fragment occurs by means of (a) DNA polymerase I (b) DNA polymerase III (c) DNA ligase (d) DNA topoisomerase (e) Primase
- How would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymeraseList and describe the function of the ten subunits constituting DNA polymerase III. Distinguish between the holoenzyme and the core enzyme.What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase