Four lab groups completed the experiment listed in the lab diluting lul of FCF dye into 1ml of water. The A625 values are listed below. For brilliant blue FCF, ɛ =97000 M-1cm–1 MW = 792.8 g/mol Group # A625 % solution (in original stock sample) Percent ror (True value of Stock=0.2%) 1 0.244 0.199 0.5 0.209 0.170 15 3 0.306 0.250 25 4 0.187 0.153 0.235 a. Which group was more accurate? b. What are possible reasons group 3 had a much higher Absorbance than the others? c. What are possible reasons group 4 had a much lower Absorbance than the others?
Q: What is the charge of an ionized peptide in the mass spectrometer if its isotopic peaks are 0.20 m/z…
A: Hi! since you haven't mentioned which question to be answered, we will answer one of the question…
Q: As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml…
A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…
Q: Louie performed paper chromatography to identify which among the four available reference mixtures -…
A: The technique of separating substances based on the differential migration across sheets of paper is…
Q: The following Rf values were computed from a reverse phase chromatography experiment. Arran them…
A: There are different techniques to separate a compound from a mixture of substances. The different…
Q: 8 7- 6- 2- 1 405 180 225 270 315 [Substrate] (nM) 45 90 135 360 450 495 540 58! What are the units…
A: Solution Michaelis menten kinetics is one of the best models of enzyme kinetics. Value of…
Q: The nitrite in a series of standard solutions (mg/L n = 5) are converted to azo dye and the slope of…
A: A standard curve is made to ensure the correct determination of the unknown concentration of the…
Q: In a neutral solution, the [H] is : O 1.0x10-14M zero 1.0×107M 1.0x10-7M
A: The acidity of any solution is determined by the molarity of the Hydrogen ions. So, the pH of any…
Q: relative density of the insoluble powder
A:
Q: Prepare the following solutions 1. 250.0 mL 0.0100 M working EDTA solution from 0.1000 M EDTA 2.…
A: a. Most of the reagents that are required are available in solutions that need to be purchased or…
Q: You purchase a premix flour containing iron (Fe), riboflavin, niacin, thiamin, and folate. For…
A: Atomic absorption spectrometry (AAS) is a simple, high-throughput, low-cost technique for analyzing…
Q: You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock…
A: Sodium chloride (NaCl) is a salt made of an equal composition of sodium and chloride ions. NaCl is…
Q: In a pharmaceutical industry a drug Paracetamol (151.163 g/mol) is determined in a sample. A 0.0315…
A: Absorbance is the crucial method of knowing the concentration of a component in a solution. It is…
Q: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950…
A: The Bradford assay is quick and requires roughly the same quantity of protein as the Lowry assay.…
Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4…
A: The Biuret test is a chemical test that is used to determine the presence of a peptide bond. In the…
Q: When 0.2 millimoles of Y are dissolved in 250 ml of water, the resultant solution has an absorbance…
A: Beer-lambert law states that for the given material, the sample concentration and path length are…
Q: Standard Curve 1.4 1.2 y = 12.235x R = 0.97758 0.8 0.6 0.4 0.2 0.02 0.04 0.06 0.08 0.1 0.12 BSA (mg)…
A: In the given question, we are given a straight line graph between Absorbance at 595 nm versus BSA…
Q: The BSA stock solution from the previous problem was then diluted to generate a set of standard…
A: CONCENTRATION OF BSA (mg/ml) ABSORBANCE 0 0 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8…
Q: What is the net ionic equation for the following chemical reaction? Pb(NO3)2(aq) + 2 NaCl(aq) → 2…
A: The net chemical equation for a reaction that lists only those species participating in the…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml)…
A: Biuret is a compound that formed when urea is warmed, which makes two urea particles to join. Urea…
Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 0 1 2 3 4…
A: The biuret assay can be used for quantitative determination of total protein concentration by…
Q: BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250…
A: A standard curve is defined as a calibration curve that is used for quantitative estimations. In…
Q: How many grams of NaOH would you need to make 3,982 mL of a 3 M NAOH solution (m.w. = 40.00 g/mol)?…
A: By the concept of molarity, we can determine the concentration of a solution. It is the total number…
Q: Given: 2KI +Pb(NO3)2 --> PbI2 + 2KNO3. How many grams of lead (II) iodide are produced if 0.250…
A: 2KI +Pb(NO3)2 --> PbI2 + 2KNO3 Thus, 2moles of KI with 1mole of Pb(NO3)2 gives 1 mole of PbI2…
Q: You want 235mL of a solution that consists of 10% BSA, 2.37mM KCl and 120µM EDTA. You have stock…
A: BSA is a globular proteins which is a well studied proteins and used as standard protein molecule in…
Q: For the following reaction 3 experiments have been run and the data collected is in the following…
A: a)
Q: If the equation produces from data is y = 0.300x - 0.857 and the absorbance for the unknown is…
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530…
A: Biuret test is a chemical test used for detecting the presence of peptide bonds, in whose presence…
Q: The iodine contained in every one mL of the 0.010 M iodine solution in the buret will react with…
A: Titration is a common laboratory method of quantitative chemical analysis to determine the…
Q: The Ksp values of silver chromate Ag2CrO4 and silver iodate Ag(IO3) are given below.…
A: Given : Ag2CrO4 Ag(IO3) Ksp…
Q: A manufacturer wishes to produce a batch of compressed tablets each containing 800 mg active…
A: A drug is a chemical substance that is used to treat any pathogenic, physiological or psychological…
Q: 4. A 1.0005 g sample contains selenite was determined using Fajan's method by performing a titration…
A: The correct option is (d). 1.372%
Q: A 75.0 g piece of Ag metal is heated to 80.0 C and dropped into 50.0 g of water at 23.2 ° C. The…
A: To find: Specific heat of silver
Q: Please help me find the structure based on this IR graph and a boiling point of 90-100°C. 84 80 70-…
A: Different types of spectroscopy can be used to identify the structure of the unknown compound. Some…
Q: Scenario: First, 0.1 mL of the unknown is mixed with 0.1 mL of distilled water. Then, 0.1 mL of the…
A: Beer-Lambert Law is applied in absorption spectrometry for biochemical analysis and measurement…
Q: Lisa and Jisoo wanted to prepare 150 mL of 0.650 M malonate buffer pH 5.00 by mixing NaC4H304 and…
A: Buffers are aqueous solutions that are made up of weak acid and its conjugate base or a weak base…
Q: 5b. Weight of 20 tablets (in mg) selected randomly is given below: Tablet no Weight (mg) Tablet…
A: To answer first we have to find the uniformity of weight of tablets. This involves calculation of…
Q: Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of…
A: Spectrophotometry enables us to calculate the concentration of a species in a solution by measuring…
Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml)…
A: The Bradford Standard curve have BSA (Bovine Serum Albumin ) concentration in X-axis and Absorbance…
Q: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 2 0.420 3 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950…
A: The Bradford Assay is is a sensitive assay to detect and quantify protein in solution.
Q: The precipitation of three solid compounds: X, Y, Z, have the following equilibrium constants: Kx =…
A: X,Y,Z Kx= 1.7×10^4 Ky= 0.6× 10^8 Kz= 9.2×10^12 As it can be seen,Kz>Ky>Kx The solubility…
Q: A solution contains 0.25 M Ni(NO3)2 and 0.25 M Cu(NO3)2. A. Can the metal ions be separated by…
A: Sodium carbonate is generally while dissolved in water, sodium carbonate forms carbonic acid and…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…
A: Even when the concentration of BSA (Bovine Serum Albumin) is 0 , an absorbance of 0.158 was…
Q: In a titration experiment, potassium iodate serves as the primary standard. Potassium iodate (KIO3)…
A: Potassium iodate (KIO3) - with 99.4% purity, weight 0.1498 g in 100 mL solution Molarity = number of…
Q: show the complete solution and write answers in 3 significant figures. DYE CALCULATED Rf Tartrazine…
A: Introduction The retention factor (Rf) of a particular compound is the ratio of the distance the…
Q: An analysis of city drinking water for total hardness was done by two students in the laboratory and…
A: Hardness of water is defined as the amount of dissolved calcium and magnesium in the water which is…
Q: The order is for 200 mg of drug to be administered IM. The directions on a vial containing 1 g of…
A: Calculating medications is an important part of nursing practice. Using the available dose, the…
Q: You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) 595nm…
A: Bradford assay is a quantitative method to determine the total protein concentration in any sample…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy…
A: The Biuret test is used to find the amount of protein in the given analyte as it checks the presence…
Q: You are a graduate student who is trying to conduct an experiment. For this experiment, you need to…
A: In order to make working solutions, it is often required to dilute the available stock solutions.…
Q: For the same chromatographic column and experimental conditions Rs when H = 3 cm = Rs when H = 5 cm…
A: Asked : For same chromatographic column and experiment conditions the answer out of given options is…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.In a neutral solution, the [H*] is : * 1.0x10-14M zero 1.0×107M O 1.0x10-7MYou wish to determine the %concentration of a stock solution of brilliant blue FCF (MW=792.8grams/mole; extinction coefficient,25=97000M1cm 1). You take 5ul of the stock and dilute up to 1ml. You take 10ul of this dilution an dilute it up to 500ul. Finally you take 1ul from this dilution and dilute it up to 1ml in a 1cm diameter cuvette. You obtain an As91=0.001 SHOW YOUR WORK. Do not forget units. 1. What is your total dilution factor? 2. What is the molar concentration of the dye in the original stock? 3. What is the % concentration of the dye in the original stock?
- Rx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMESA flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]
- BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.You just made a 1.5M permanganate solution. What concentration is your potassium permanganate solution in percent? "K: 39.10 g/mole" "Mn: 54.94 g/mole" "O: 16 g/mole" "MW of KMnO4 is 158.04 g/mole" O a. 0.24% Ob. 20.1% O c. 15.8% O d. 23.7% O e. 2%The Ksp values of silver chromate Ag2CrO4 and silver iodate Ag(IO3) are given below. Ag2CrO4 Ag(IO3) Ksp 1.12 x 10-12 3.17 x 10-8 Based on these Ksp values, which of the following is true? Choose one option only. Options: a. In the solution consisting of 1.00 x10-4 M Ag+ and 5.00 x10-5 M CrO42-, Ag2CrO4 precipitate will form. b. In the solution consisting of 1.0 x10-4 M Ag+ and 1.0 x10-4 M IO3-, Ag(IO3) precipitate will form. c. In pure water, the solubility of Ag2CrO4 is lower than the solubility of Ag(IO3). d. In the solution consisting of 0.200 M CrO42- and 0.200 M IO3-, Ag2(CrO4) will precipitate first if we add Ag+ ions gradually into the above mixture.
- You are tasked to prepare 1000mL of 2 media preparations, however, instead of using a powder agar, you will be preparing the desired concentration from a prepared stock solution. Complete the information in the table and show your computation. Volume of Volume of diluent Desired Stock Solution stock sol'n needed to make Concentration needed 1000mL media 25% 12.5% 5% 1.5%Lead nitrate Stock Solution— Dissolve 159.8 mg of lead nitrate in 100 mL of water to which has been added 1 mL of nitric acid, then dilute with water to 1000 mL. Standard Lead Solution— On the day of use, dilute 10.0 mL of Lead Nitrate Stock Solution with water to 100.0 mL. Standard Preparation— Into a 50-mL color-comparison tube pipet 2 mL of Standard Lead Solution, and dilute with water to 40 mL, and mix. Test Preparation— Into a 50-mL color-comparison tube dissolve 1.0g ascorbic acid (mol wt 176.12) in 25 mL of water, dilute with water to 40 mL, and mix. Procedure— To each of the two tubes containing the Standard Preparation, the Test Preparation, add 2 mL of pH 3.5 Acetate Buffer, then add 1.2 mL of thioacetamide–glycerin base TS, dilute with water to 50 mL. Mix, allow to stand for 2 minutes, and view downward over a white surface. The color of the solution from the Test Preparation is not darker than that of the solution from the Standard Preparation. The colour is formed when…Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…