If PBR322 is digested simultaneously with Pstl and BamHI, i. How many fragments are created? ii. Write down the base pairs that result at the 5' and 3' ends of the shortest fragment. HindlII EcoRI | ECORV BamHI 4000 375 PstI, Ijes tet amp 1000 PBR322 4361 bp ori 2295 Ndel

Q: 1.0 MS2 T4 E. coli 0.5 104 103 102 10- 10' 102 103 10* 1 Cot (mole x sec/L) b.) This next figure…

A: he cot curve is depicted in the diagram above. It explains the process of dentauration. The double…

Q: How  big (in base pairs)  is (are) the fragments generated by digesting the vector with EcoRI

A: Base pairs - there are four bases or nucleotides in DNA: ADENINE, CYTOSINE, GUANINE, and thymine.

Q: Refer to the attached DNA model.  In the spaces provided on the attached table, identify the…

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Q: The total (50ul) of your restriction digest contains 500ng of DNA how much DNA (ng) are you loading…

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Q: If pET32a(+) is digested simultaneously with SacI and Bc1I 1. How many fragments are created? 2.…

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Q: The sequence below (A) was read from the autoradiogram (B). (A) 5'…

A: Note: since you have asked multiple unrelated questions, as per the honor code we are answering the…

Q: The restriction endonuclease PstI cuts DNA symmetrically on both strands at the sequence: CTGCAG…

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Q: pMDawn is digested with EcoR1, and BamHI. Resulting in fragments shown below: EcoRI: 20 kb BamHI:…

A: Given: Digestion of a plasmid PMDawn with EcoRI, BamHI, and EcoRI+BamHI are as follows: EcoRI: 20 kb…

Q: The 50ul of your restriction digest contains 500ng of DNA how much DNA (ng) are you loading into the…

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Q: The sequence of the 15 bp fragment from the previous problem is repeated below: 5' TCTGAATTCCGTAGA…

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Q: When running a gel during a Sanger sequencing, the following results were obtained: I I Find the DNA…

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Q: Ladder LS KT MV DY JY Ladder 850 bp 550 bp 11 1

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Q: DNA renaturation curves occasionally show three distinct phases of renaturation. In this graph, DNA…

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Q: Sall Kpnl Kpnl 450 600 200 400 1650 1. If you were to subject this digested DNA to agarose gel…

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Q: If pET32a(+) is digested simultaneously with SacI and BclI i) How many fragments are created?…

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Q: If pET32a(+) is digested simultaneously with BamHI and BclI, 5.1.  How many fragments are created?…

A: pET-32 is a unique sequence outlined for cloning and high-standard peptide sequences expression.

Q: If pET32a(+) is digested simultaneously with BamHI and BclI, 1.How many fragments are created? 2.…

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Q: By average, how many Sau3A (5’GATC3’) sites are there in a 10 kd DNA molecule? (1/4)^6 * 10,000 =…

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Q: Given the banding pattern from the gel electrophoresis below, the nucleotide sequence of this…

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Q: The λ DNA used for digestion was provided at a concentration of 2μg/μl. How much DNA (in micrograms)…

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Q: You are studying a genome that has 42% G:C content and the remaining 58% of the genome is As and Ts.…

A: Restriction enzymes  The enzyme that is use to cut the DNA at specific site.

Q: Which lane shows the DNA fragment completely digested with Pst n

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Q: BamHI Haell Both 10 kb 9 kb 8 kb 5 kb 2 kb 1 kb Figure 2 (i) How long is the original DNA molecule?…

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Q: Given the banding pattern from the gel electrophoresis below, the nucleotide sequence of this…

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.