2 In addition to cleaving peplides, chymotrypsin can also catalyze the Irydrolysis of certain smalorganic molecules such as acetanīlides (below).Based on the data given below, which compound, 4-melhyl- or 4-chloroacelanilide, is the better substrate(L.e., the more specific substrate) for chymotrypsin? Briefly explain how the data indicate that this amide isthe better substrate for the enzyme.CompoundX= CH,Ka (min")5.22K. (mM)13X= CI0.840.67As wilh peptides, chymolrypsin-catalyzed hydrolysis of acetanilides uses the hydroxyl group of a serine toform an intermediate containing a covalent bond between the enzyme and a portion of the substrate.Using E-OH to represent the enzyme, draw the structure of the covalent enzyme-intermediate complex forthe reaction of chymotrypsin with acetanilide (Ph = benzene ring).

Hi! Thank you for the question. We are authorized to answer one question at a time, since you have…

Answered: In addition to cleaving peplides,…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Will the synthesis of 9,10-dihydroanthracene-9,10-α,β-succinic anhydride via Diels-Alder reaction occur faster under photochemical conditions? Explain.
In the first step of the aldolase reaction, an active site Lys229 residue, with its side chain amino group in the deprotonated state, acts as a nucleophile and attacks the carbonyl C2 carbon of fructose 1,6-bisphosphate to form a Schiff base (boxed in the scheme). Since the pKa of the Lys side chain amino group in free solution is ~10.5, the pKa of Lys229 side chain must have been perturbed to a (higher lower) value for the enzyme to be active at neutral pH. the answer should include sufficient details, including the definition of pKa.
Is it possible to produce derivatives of superoxide dismutase that have either copper in all the metal sites (that is, copper replacing zinc) or zinc in all the metal sites (zinc replacing copper)? Would you expect either of these derivatives to be active? What would you expect for the magnetic properties of the two derivatives?
With the help of the half reactions given in Table 1, formulate the redox equation for the oxidation of succinate and reduction of ubiquinone.
The mechanism involved in the reaction catalyzed by phosphoglyceromutase is known to involve a phosphorylatedenzyme intermediate. If 3-phosphoglycerate is radioactively labeledwith 32P, the product of the reaction, 2-phosphoglycerate, does nothave any radioactive label. Design a mechanism to explain these facts.
Considering the general structure of penicillins, how are they rendered ineffective? Select one: a. β-Lactamase performs nucleophilic attack of the amide in the penicillin side chain b. β-Lactamase performs nucleophilic attack of the amide in the β-lactam ring and subsequently hydrolyzes this intermediate c. β-Lactamase performs nucleophilic attack of the thiazolidone ring on the carbon next to the electronegative sulfur leading to a covalently inhibited β-Lactamase d. All of these e. None of these
The turnover number of the enzyme fumarase that catalyzes the reaction, Fumarate + H20 ===→ L-malate, is 2.5 x 103 S - l and Km = 4.0 X 10- 6 mol/L. Calculate the rate of conversion of fumarate to L-malate if the fumarase concentration is 1.0 x 1 0 - 6 mol/L and the fumarate concentration is 2.04 x 10- 4 mol/L.
An inhibitor that specifically labels chymotrypsin at histidine 57 is N-tosylamido-l-phenylethyl chloromethylketone. How would you modify the structure of this inhibitor tolabel the active site of trypsin?
What are the measures to inhibit the Maillard reaction in undesirable situations. please explain detailed
Gentiobiose has the molecular formula C12H22O11 and has been isolated from gentian root and by hydrolysis of amygdalin. Gentiobiose exists in two different forms, one melting at 86°C and the other at 190°C. The lower melting form is dextrorotatory ([α] 16°), the higher melting one is levorotatory ([α] -6°). The rotation of an aqueous solution of either form, however, gradually changes until a final value of [α] 9.6° is observed. Hydrolysis of gentiobiose is efficiently catalyzed by emulsin and produces two moles of D-glucose per mole of gentiobiose. Gentiobiose forms an octamethylether, which on hydrolysis in dilute acid yields 2,3,4,6-tetra-O-methyl-D-glucose and 2,3,4-tri-O-methyl-D-glucose. What is the structure of gentiobiose? EXPLAIN IN DETAIL.
Ascorbic acid: What does it mean to say that ascorbic acid is a strong reducing agent? What is the active form of ascorbic acid in plasma and how is it formed? How can we distinguish the various oxidation states of ascorbic acid? Using chemical symbols or structures, illustrate the reducing activity of ascorbate in forming ascorbyl radical and dehydroascorbic acid Why do we refer to the ascorbate/ascorbyl-radical/dehydroascorbate system as a reversible redox system? Explain the role of ascorbic acid in： Collagen synthesis Iron absorption Regeneration of a-tocopherol
Give the pharmacological property of the following terpenes/terpenoids. Where do they naturally occur? saponins terpenoids cardiac glycosides How can terpenes/terpenoids be extracted from plants? What are the chemical tests used in identifying the presence of cardiac glycosides?

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS