Incorrect The mechanism by which DNA is licensed to replicate only once during each S phase involves ORC and helicase loaders binding to geminin. MCM proteins binding to geminin after replication ORC and helicase loaders being phosphorylated. MCM proteins binding only to replicated DNA. MCM proteins binding only to CEN DNA. 0/1 pts
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- Which of the following enzymes remove supercoiling in replicating DNA ahead of the replication fork?a) DNA polymerasesb) Helicasesc) Primasesd) TopoisomerasesA gene encoding one of the proteins involved in dna replication has been inactivated by a mutation in a cell. in the absence of this protein, the cell attempts to replicate its dna. What would happen during the dna replication process if each of the following proteins were missing?a. DNA polymerase B. DNA ligasec. Sliding clamp for DNA polymerased. nuclease that removes RNA primerse. DNA helicase F. primaseAt a growing replication fork, the replication of the two strands is: Question 15 options: continuous for the leading strand and discontinuous for the lagging strand. continuous for the lagging strand and discontinuous for the leading strand. uncoupled and independent of each other. discontinuous for both strands due to low processivity of the DNA polymerase.
- Using the correct base pairing rules for DNA replication, what would be the complementary strand for the strand TACCGATGC? Multiple Choice TACCGATGC ATGGCTACG None of the answer choices are correct. GCTAGTCGA TTCCGTTCGWhich of the following reactions is required for proofreading during DNA replication by DNA polymerase III?a) 5’ to 3’ exonuclease activityb) 3’ to 5’ exonuclease activityc) 3’ to 5’ endonuclease activityd) 5’ to 3’ endonuclease activityHow would nucleotide excision repair be affected if one of the followingproteins was missing? Describe the condition of the DNAif the repair was attempted in the absence of the protein.A. UvrAB. UvrCC. UvrDD. DNA polymerase
- Assume a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced. What will be the most likely consequence of this mutation? The DNA would not exist in a supercoiled state. There would be no RNA primers laid down. The DNA will not be able to unwind to initiate replication. The DNA strands would contain pieces of RNA. There would be no DNA replication on the leading or lagging strands.Polymerases work is to add 10 nucleotides to a DNA strand before dissociating. During replication process, DNA pol III can add tens of thousands of nucleotides at a moving fork. How this additionaccomplished?Translesion synthesis (TLS) polymerase zeta (ζ) is capable of accurately reading through 6,4-photoproducts (cytotoxic damage). If this protein is non-functional: a different TLS polymerase will accurately read through the DNA lesion. a different TLS polymerase will inaccurately read through the DNA lesion resulting in increased mutational frequency. the replicative polymerase will inaccurately read through the DNA damage. DNA polymerase alpha (α) will be recruited to synthesize a primer across the DNA damage. the DNA lesion cannot be bypassed and will result in double stranded DNA breaks.
- Which of the following statements about replication is false? The proofreading function of DNA polymerases involves 3’ 5’ exonuclease activity. Origins of replication are rich in G-C nucleotide pairs. A characteristic of aging cells is that telomeres become shorter. The lagging strand is synthesized in the opposite direction as the movement of the replication fork. DNA polymerase is more accurate that RNA polymerase.Replication slippage can cause indels in repetitive regions of DNA. Which of the following statements is not true?a) slippage of the new daughter strand causes repeat expansionb) slippage of the template strand causes repeat deletion c) slippage can cause indels on either the daughter or the template strandd) the indel mutation becomes fixed in one daughter cell's genome only after the next round of replication e) none of the above (all are true statements)Which of the following statements about the DNA replication is false? a. Synthesis of the new DNA strands is form 39 to 59 b.Synthesis of the new DNA strands is from 59 to 39 c. DNA Unwinds, primase adds RNA primer, DNA polymerases synthesize the new strand and remove the RNA primer d. Many initiation points exist in each eukaryotic chromosome. e. Okazaki fragments are synthesized in the opposite direction from the direction in which the replication for moves.