Introduce and explain comprehensively the process of calculating the final magnification of specimens using a compound microscope. What is scanning objective and why it should be located at the beginning of the process?
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Introduce and explain comprehensively the process of calculating the final magnification of specimens using a compound microscope. What is scanning objective and why it should be located at the beginning of the process?
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- Discussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?What factors must be addressed to properly acquire quantitative fluorescence microscopy images? What information about the imaging system should be recorded? What controls are needed and what standards are available?How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensity
- What are the differences between scanner, low power objective, high power objective and oil immersion objective? Introduce the terms stated above and explain comprehensively.What’s the aim of using a microscope for examining? What can the kohler illumination technique tell us? What are the sources of error that can impact the data ?In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?
- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?briefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)
- 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?Explain in detail simple and differential staining and also improving and adjusting contrast in light microscopy.Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
